公开(公告)号：US20190017041A1

公开(公告)日：2019-01-17

申请号：US16042313

申请日：2018-07-23

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Scott BANTA ,  Mark A. BLENNER ,  Ian WHEELDON ,  Kevin DOOLEY

IPC分类号： C12N9/88 ,  A61L15/44 ,  A61L27/22 ,  A61L15/60 ,  A61L15/46 ,  A61L15/42 ,  A61L15/32 ,  A61L27/54 ,  A61L27/52 ,  A61L27/50 ,  C08L89/00

摘要： In one aspect, the invention relates to a peptide that forms a calcium-dependent hydrogel using a rationally engineered beta roll peptide. In the absence of calcium, the peptide is intrinsically disordered. Upon addition of calcium, the peptide forms a corkscrew-like structure. In one embodiment, one face of the beta roll is mutated to comprise leucine residues. In some embodiments, a leucine zipper forming helical domain to the engineered beta roll forms hydrogels by physical cross-linking in calcium rich environments.

公开(公告)号：US20160152966A1

公开(公告)日：2016-06-02

申请号：US14814102

申请日：2015-07-30

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Scott BANTA ,  Mark A. BLENNER ,  Ian WHEELDON ,  Kevin DOOLEY

IPC分类号： C12N9/88

CPC分类号： C12N9/88 ,  A61L15/32 ,  A61L15/42 ,  A61L15/44 ,  A61L15/46 ,  A61L15/60 ,  A61L27/227 ,  A61L27/50 ,  A61L27/52 ,  A61L27/54 ,  A61L2400/06 ,  C12Y406/01001 ,  C08L89/00

摘要： In one aspect, the invention relates to a peptide that forms a calcium-dependent hydrogel using a rationally engineered beta roll peptide. In the absence of calcium, the peptide is intrinsically disordered. Upon addition of calcium, the peptide forms a corkscrew-like structure. In one embodiment, one face of the beta roll is mutated to comprise leucine residues. In some embodiments, a leucine zipper forming helical domain to the engineered beta roll forms hydrogels by physical cross-linking in calcium rich environments.

公开(公告)号：US20240060199A1

公开(公告)日：2024-02-22

申请号：US18270560

申请日：2021-12-29

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Jonathan VARDNER ,  Alan WEST ,  Scott BANTA

IPC分类号： C25C1/12

CPC分类号： C25C1/12

摘要： A copper concentrate such as chalcopyrite is contacted with an aqueous solution includes acids and a reducing agent, such as vanadium (II) ions, chromium (II) ions, or tungstozincic acid (H6ZnW12O40). The aqueous solution reduces the copper in the copper concentrate, which can then dissolve into the solution for recovery therefrom, or precipitate out of solution as copper compounds or elemental copper for recovery in as a solid phase product. The solid phase product can then be isolated, dissolved, and further electrowinned to recover a copper product from the copper concentrate. Oxidized reducing agent can be recovered in an electrochemical device with ferrous iron reactants. Hydrometallurgical routes to convert copper concentrates to copper are potentially less expensive and less polluting than current pyrometallurgical processing and an advantageous response to environmental and economic pressures for increased copper production.

公开(公告)号：US20220033985A1

公开(公告)日：2022-02-03

申请号：US17390087

申请日：2021-07-30

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Alan WEST ,  Scott BANTA ,  Jon VARDNER ,  Campbell DONNELLY ,  Zhengyan ZHANG

IPC分类号： C25B15/08 ,  C25B1/01 ,  C25B9/40 ,  C25B11/037 ,  C25B11/042 ,  C25B13/02 ,  C22B15/00

摘要： An electrochemical system and process are provided to convert an amount of chalcopyrite (CuFeS2) to a product including copper ions. In an electrochemical reactor, a potential is applied across an anode and a cathode to convert the chalcopyrite to an intermediate, chalcocite (Cu2S). The anode is covered to prevent contact with the intermediate, thus limiting subsequent conversion of the intermediate to covellite (CuS) in favor of conversion to a material more suited to chemical oxidation, cuprite (Cu2O). For example, the anode can be covered with one or more layers of filter paper. Upon application of an oxidizing agent, the cuprite is oxidized to produce a product including copper ions. The cathode and covered anode allow for efficient and inexpensive processing. The cost of this technique is comparable to industry standards, and moreover, has a much smaller environmental footprint than heat-based copper extraction.

公开(公告)号：US20240318218A1

公开(公告)日：2024-09-26

申请号：US18610436

申请日：2024-03-20

申请人： The Trustees of Columbia University in The City of New York

发明人： Scott BANTA ,  Emma WILLETT

IPC分类号： C12P19/30

CPC分类号： C12P19/30

摘要： Synthetic enzymatic cascades are provided that continuously produce adenosine triphosphate (ATP) from a variety of fuel sources. The cascades are prepared by expressing one or more NADH-dependent dehydrogenases, polyphosphate NAD+ kinases (PPNK), NADPH oxidases, and particular reversible ATP-NAD+ kinases (NADK). The NADH-dependent dehydrogenases oxidize fuel sources such as formate and methanol while converting NAD+ to NADH. The PPNKs convert the NADH to NADPH. The NADPH oxidases convert NADPH to NADP+. The NADKs then convert the NADP+ to NAD+ while also facilitating conversion of adenosine diphosphate (ADP) or adenosine monophosphate (AMP) to ATP. Human NADK exhibits high affinity for NAD+ and is thus impeded in the generation of ATP products via product inhibition. Thus, pigeon, duck, and cat NADK isoforms are implemented in the cascade instead. The cascades generate a low-cost, continuous source ATP product for use in numerous in vitro applications such as cell-free protein production.

公开(公告)号：US20240209472A1

公开(公告)日：2024-06-27

申请号：US18124822

申请日：2023-03-22

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Scott BANTA ,  Farid KHOURY

IPC分类号： C22B3/18 ,  C22B59/00

CPC分类号： C22B3/18 ,  C22B59/00

摘要： Rare earth elements (REEs) are recovered by dissolving an REE-containing source in one or more solvents. The resulting solution is contacted with block V repeats-in-toxin (RTX) domains of adenylate cyclase from Bordetella pertussis. These polypeptides are generally intrinsically disordered. However, upon binding an amount of the REEs and/or REE-containing compounds, the polypeptide folds to form a beta roll (BR) secondary structure. The polypeptides also adopt the BR structure at very low pH, e.g., below about 1.5, yet are still capable of effectively binding REEs. The metal-peptide constructs can then be isolated for recovery of REE products. Native and synthetic RTX/BR domains can be used to bind and recover REEs with higher binding capacity than lanmodulin and enhanced REE selectivity. For example, embodiments of the present disclosure can be used to extract REEs from electronic wastes such as NdFeB magnets, seaweed ashes, used during biomining and bioleaching operations, etc.

公开(公告)号：US20140187746A1

公开(公告)日：2014-07-03

申请号：US14052367

申请日：2013-10-11

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

发明人： Scott BANTA ,  Oren SHUR

IPC分类号： C07K1/30 ,  C07K7/06

CPC分类号： C07K1/303 ,  C07K1/30 ,  C07K7/06 ,  C07K14/245 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/24 ,  C07K2319/50 ,  C07K2319/92

摘要： The invention is directed to a Ca2+ precipatable polypeptide tags and cassettes useful for purification of molecules from heterogeneous samples. The invention also relates to methods for bioseparation of molecules comprising Ca2+ precipatable tags and cassettes.

摘要翻译： 本发明涉及可用于从异质样品纯化分子的Ca 2+可沉淀多肽标签和盒。 本发明还涉及用于生物分离包含Ca 2+可沉淀标签和盒的分子的方法。