公开(公告)号：US12188015B2

公开(公告)日：2025-01-07

申请号：US17254169

申请日：2019-06-21

Applicant: Cornell University ,  The Regents of the University of Michigan

Inventor： Ailong Ke ,  Adam Dolan ,  Yan Zhang ,  Zhonggang Hou

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: Provided are compositions, methods, and kits for CRISPR-based editing of DNA targets by Type I CRISPR-associated (Cas) enzymes.

公开(公告)号：US20240229081A1

公开(公告)日：2024-07-11

申请号：US18563175

申请日：2022-05-26

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN ,  CORNELL UNIVERSITY

Inventor： Yan Zhang ,  Zhonggang Hou ,  Renke Tan ,  Ailong Ke

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure provides systems and methods of altering a nucleic acid sequence, which comprise an engineered Type I CRISPR/Cas system comprising Cas3 and Cas11. Particularly. the system and methods described herein use compact engineered CRISPR-Cas3 systems (e.g., Type I-C, Type I-B, or Type I-D) for genetic manipulations (e.g., in eukaryotic cells).

公开(公告)号：US20230287457A1

公开(公告)日：2023-09-14

申请号：US18000676

申请日：2021-05-26

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN ,  CORNELL UNIVERSITY

Inventor： Yan Zhang ,  Ailong Ke ,  Ryan Krueger ,  Zhonggang Hou

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N15/70

CPC classification number: C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  C12N15/70 ,  C12N2310/20 ,  C12N2800/80

Abstract: The disclosure provides a method of altering a DNA sequence, which method comprises the use of a type I-C CRISPR-Cas system based on Neisseria lactamica. The system involves a combination of two or more Neisseria lactamica proteins selected from Cas3, Cas5, Cas8c, and Cas7 and a synthetic guide RNA sequence.