公开(公告)号：US20240191192A1

公开(公告)日：2024-06-13

申请号：US18554372

申请日：2022-04-07

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED ,  NOILE-IMMUNE BIOTECH INC.

Inventor： Soichiro OGAKI ,  Hideo ARAKI ,  Eiki MAEDA

IPC: C12N5/0783 ,  C07K16/28

CPC classification number: C12N5/0636 ,  C07K16/2809 ,  C07K16/2818 ,  C12N2510/00

Abstract: Provided is a method for activating T cells comprising the step of activating T cells in a medium containing a CD3 agonist and/or a CD28 agonist for more than 36 hours and less than 48 hours; a method for producing genetically modified T cells, comprising the steps of activating T cells according to the activation method, introducing an exogenous gene into the activated T cells, and culturing the T cells into which the exogenous gene has been introduced; a cell population comprising genetically modified T cells obtained by the production method; and a matrix of mobile polymer chains or a bead supporting a CD3 agonist and/or a CD28 agonist, wherein the matrix or bead is used for being added to a medium and subjecting T cells to an activation step for more than 36 hours and less than 48 hours.

公开(公告)号：US20250052740A1

公开(公告)日：2025-02-13

申请号：US18722919

申请日：2022-12-22

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Soichiro OGAKI ,  Tomomine IIDA ,  Shogo SAITO ,  Hideo ARAKI

IPC: G01N33/50

Abstract: Disclosed is a method for predicting the gene transfer efficiency of animal cells, comprising (1) measuring the size of transgenic animal cells, and (2) predicting the gene transfer efficiency based on values measured in step (1).