ANALYSIS METHOD, ANALYSIS SYSTEM, AND ANALYSIS SURFACE

    公开(公告)号:US20230066282A1

    公开(公告)日:2023-03-02

    申请号:US17760350

    申请日:2021-02-04

    Abstract: An object is to provide a method capable of associating position information of a cell with a cell constituent at a single-cell resolution. That is, the present technology provides an analysis method including: an imaging step of imaging a specimen in a state in which the specimen is being overlapped with a surface to which a molecule having a linker cleavable by stimulation, a barcode sequence, and a target capture portion is immobilized via the linker; an association step of associating a position of a cell with the barcode sequence of the molecule at the position by using a specimen image obtained by the imaging; a cleavage step of selectively stimulating the position of the cell to cleave the linker of the molecule at the position; and a binding step of binding the molecule released from the surface by the cleavage with a constituent of the cell via the target capture portion of the molecule.

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