公开(公告)号：US20230349847A1

公开(公告)日：2023-11-02

申请号：US18307936

申请日：2023-04-27

Applicant: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY CHINESE ACADEMY OF SCIENCES

Inventor： Horst VOGEL ,  Zhili RAO ,  Xueying WANG ,  Xiaokang ZHANG ,  Shuguang YUAN ,  Huawei ZHANG

IPC: G01N23/2251 ,  G01N23/2202

CPC classification number: G01N23/2251 ,  G01N23/2202

Abstract: A method for determining a protein structure using cryo-electron microscopy, including: enabling a target protein to contain a tag; enabling a resulting target containing the tag to bind a scaffold protein to form a complex between the target protein and the scaffold protein; and performing single-particle imaging using the cryo-electron microscopy to determine a structure of the target protein in complex with the scaffold protein. The scaffold protein is any one of streptavidin, avidin, or derivatives thereof. The tag is configured for selectively binding to the scaffold protein. The tag is one selected from the group consisting of: a biotin tag, comprising a biotin; a biotinylated protein or polypeptide tag, comprising a protein sequence and a biotin covalently linked to the protein sequence; a Strep-tag; and a biotinylated or strep-tagged antibody, or antibody Fab fragment, or single-chain antibody.