公开(公告)号：US12098379B2

公开(公告)日：2024-09-24

申请号：US17723806

申请日：2022-04-19

申请人： SHANDONG AGRICULTURAL UNIVERSITY

发明人： Fei Ni ,  Daolin Fu ,  Juan Qi ,  Jiajie Wu

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8289 ,  C07K14/415

摘要： A barley male sterility gene HvMSG47, wherein the gene HvMSG47 is (i) a nucleotide sequence as set forth in SEQ ID NO: 1. (ii) a nucleotide sequence as set forth in SEQ ID NO: 2, or (iii) a nucleotide sequence that has 90% or more homology with the nucleotide sequence of (i) or (ii) and expresses the same functional protein.

公开(公告)号：US20220073938A1

公开(公告)日：2022-03-10

申请号：US17480686

申请日：2021-09-21

申请人： SHANDONG AGRICULTURAL UNIVERSITY

发明人： Daolin Fu ,  Mincheng Luo ,  Juan Qi ,  Fei Ni ,  Bo Lv ,  Shuyun Wang

IPC分类号： C12N15/82 ,  C07K14/415 ,  C12N15/113

摘要： The present invention provides a novel gene WMS conferring wheat male sterility, its anther-specific expression promoter, and uses of the same. In wheat, a well-known gene Ms2 causing dominant male sterility has been widely applied in recurrent selection in China. A RNA-seq approach was performed to reveal the anther-specific transcriptome in a pair of Ms2 isogenic lines, ‘Lumai 15’ and ‘Lumai 15+Ms2’. As a result, a WMS gene was identified showing anther-specific expression at the early stage of meiosis and only in wheat carrying the Ms2 gene. The regulation of WMS could alter plant male fertility. The promoter of WMS was found to comprise anther-specific activity. Thus, the present invention might be used to achieve anther-specific gene expression, to develop male sterility in various plant species, to establish recurrent selection in various plant species, and to assist hybrid seed production.

公开(公告)号：US11124797B1

公开(公告)日：2021-09-21

申请号：US15579058

申请日：2016-04-11

申请人： Shandong Agricultural University

发明人： Daolin Fu ,  Mincheng Luo ,  Juan Qi ,  Fei Ni ,  Bo Lv ,  Shuyun Wang

IPC分类号： C12N15/82 ,  C12N15/113 ,  C07K14/415

摘要： The present invention provides a novel gene WMS conferring wheat male sterility, its anther-specific expression promoter, and uses of the same. In wheat, a well-known gene Ms2 causing dominant male sterility has been widely applied in recurrent selection in China. A RNA-seq approach was performed to reveal the anther-specific transcriptome in a pair of Ms2 isogenic lines, ‘Lumai 15’ and ‘Lumai 15+Ms2’. As a result, a WMS gene was identified showing anther-specific expression at the early stage of meiosis and only in wheat carrying the Ms2 gene. The regulation of WMS could alter plant male fertility. The promoter of WMS was found to comprise anther-specific activity. Thus, the present invention might be used to achieve anther-specific gene expression, to develop male sterility in various plant species, to establish recurrent selection in various plant species, and to assist hybrid seed production.