公开(公告)号：US20240200005A1

公开(公告)日：2024-06-20

申请号：US18555347

申请日：2022-04-14

Applicant: SCREENSYS GMBH

Inventor： Jialan CAO ,  Alexander GROSS ,  Johann Michael KÖHLER ,  Patrick SCHAUB ,  Klaus PALME ,  Oleksandr DOVZHENKO

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N5/04 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/00

CPC classification number: C12M23/16 ,  C12M41/46 ,  C12M47/04 ,  C12N5/04 ,  G01N15/1434 ,  G01N15/149 ,  G01N33/0098 ,  C12N2523/00 ,  C12N2529/10

Abstract: The present invention relates to a method for developing and/or reprogramming plant cellular objects comprising the steps: providing a reservoir containing a medium with plant cellular objects: providing a first set of compartments of sample fluid embedded in carrier fluid in a microfluidic conduit, wherein the carrier fluid is immiscible with the medium, wherein the first set's compartments of sample fluid each comprise medium and at least one plant cellular object: providing one or more first state triggers to the plant cellular objects in the microfluidic conduit for inducing a first state in the plant cellular objects of the first set of compartments: incubating the plant cellular objects of the first set of compartments in the microfluidic conduit for a time span sufficient for the plant cellular objects to transfer to the first state: selecting one or more first selection parameters indicative of the first state: identifying, within the first set of compartments in the microfluidic conduit, compartments according to the one or more first selection parameters and optionally assigning the compartments with respective state identifiers.