公开(公告)号：US20030104405A1

公开(公告)日：2003-06-05

申请号：US10067241

申请日：2002-02-07

申请人： SAPPORO BREWERIES LIMITED

发明人： Masahide Sato ,  Youichi Tsuchiya

IPC分类号： C07H021/04 ,  C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6895 ,  C12Q2600/158

摘要： The method of differentiating beer yeast of the invention is a method which comprises a first step of synthesizing a primer capable of amplifying the linker portion between a base sequence (A) and a base sequence (B) in a novel gene (C) which has the base sequence (B) comprising a portion of yeast chromosome IX linked downstream from the base sequence (A) comprising a portion of the N-terminal end of yeast gene Lg-FLO1, and which includes the base sequences listed as SEQ. ID. Nos. 1-6 of the Sequence Listing; a second step of carrying out a PCR (Polymerase Chain Reaction) using the primer synthesized in the first step and DNA separated from a yeast specimen; and a third step of differentiating whether the yeast is bottom-fermenting yeast or wild yeast, based on the PCR amplification product obtained from the second step.