公开(公告)号：US11021745B2

公开(公告)日：2021-06-01

申请号：US16272970

申请日：2019-02-11

申请人： Roche Sequencing Solutions, Inc.

发明人： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech

IPC分类号： G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  C12M1/34 ,  G01N27/447

摘要： This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20230416813A1

公开(公告)日：2023-12-28

申请号：US18211425

申请日：2023-06-19

申请人： Roche Sequencing Solutions, Inc.

发明人： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech

IPC分类号： C12Q1/6869 ,  G01N33/487 ,  C12M1/34 ,  G01N27/327 ,  G01N27/447

CPC分类号： C12Q1/6869 ,  G01N33/48721 ,  C12M1/34 ,  G01N27/3278 ,  G01N27/3277 ,  G01N27/44791

摘要： This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US11725236B2

公开(公告)日：2023-08-15

申请号：US17242098

申请日：2021-04-27

申请人： Roche Sequencing Solutions, Inc.

发明人： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech

IPC分类号： C12Q1/6869 ,  G01N33/487 ,  C12M1/34 ,  G01N27/327 ,  G01N27/447

CPC分类号： C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2565/631

摘要： This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.