公开(公告)号：US20210388407A1

公开(公告)日：2021-12-16

申请号：US17409158

申请日：2021-08-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： John CHEN ,  Shawn LAWRENCE ,  Amy JOHNSON ,  Theodore LONEY ,  Ravindra PANGULE ,  Ta-Chun HANG ,  Scott CARVER ,  Bernhard SCHILLING

IPC: C12P21/00 ,  C07K14/47 ,  C07K14/715 ,  C07K14/71

Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

公开(公告)号：US20200255880A1

公开(公告)日：2020-08-13

申请号：US16864689

申请日：2020-05-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： John CHEN ,  Shawn M. LAWRENCE ,  Amy JOHNSON ,  Theodore LONEY ,  Ravindra PANGULE ,  Ta-Chun HANG ,  Scott CARVER ,  Bernhard SCHILLING

IPC: C12P21/00 ,  C07K14/71 ,  C07K14/715 ,  C07K14/47

Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

公开(公告)号：US20210388408A1

公开(公告)日：2021-12-16

申请号：US17409193

申请日：2021-08-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： John CHEN ,  Shawn LAWRENCE ,  Amy JOHNSON ,  Theodore LONEY ,  Ravindra PANGULE ,  Ta-Chun HANG ,  Scott CARVER ,  Bernhard SCHILLING

IPC: C12P21/00 ,  C07K14/47 ,  C07K14/715 ,  C07K14/71

Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.