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公开(公告)号：US20220135995A1
公开(公告)日：2022-05-05
申请号：US17085860
申请日：2020-10-30
IPC分类号： C12N15/82 , C07K14/415 , C12Q1/6895
摘要： The present invention relates to an allele designated alpha-WOLF 27 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that may comprise in its amino acid sequence: a) the motif “MAEIGYSVC” SEQ ID NO: 1 at its N-terminus; and b) the motif “KWMCLR” SEQ ID NO: 2; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence similarity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:7, Pfs:8, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:17.
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公开(公告)号：US20210222190A1
公开(公告)日：2021-07-22
申请号：US17158275
申请日：2021-01-26
IPC分类号： C12N15/82 , C07K14/415 , C12Q1/6895
摘要： The present invention relates to a modified gene that provides resistance to Cucurbit Yellow Stunting Disorder virus (CYSDV) when present in a plant of the Cucurbitaceae family. The invention further relates to progeny, seed, and fruit of the plant that is resistant against CYSDV. The invention also relates to propagation material suitable for producing the plant that is resistant to CYSDV. Additionally, the invention relates to methods for producing, identifying, and selecting a plant of the Cucurbitaceae family having resistance against CYSDV.
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公开(公告)号：US11820993B2
公开(公告)日：2023-11-21
申请号：US17085897
申请日：2020-10-30
IPC分类号： C12N15/82 , C07K14/415 , A01H1/00
CPC分类号： C12N15/8282 , A01H1/1255 , C07K14/415
摘要： The present invention relates to an allele designated alpha-WOLF 26 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that may comprise in its amino acid sequence: a) the motif “MAEIGYSVC” SEQ ID NO: 1 at its N-terminus; and b) the motif “KWMCLR” SEQ ID NO: 2; and wherein the LRR domain of the protein has in order of increased preference at least 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:7, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:16, Pfs:17, and does not confer resistance to downy mildew race Pfs:8 and Pfs:9.
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公开(公告)号：US11332755B2
公开(公告)日：2022-05-17
申请号：US16738028
申请日：2020-01-09
发明人： Vincent Laurens Adrianus Kock , Johannes Geert Jan Feitsma , Raoul Jacobus Johannes Maria Frijters
IPC分类号： C12N15/82 , C12Q1/6895 , A01H5/12 , A01H6/02
摘要： The present invention relates to a method for modifying the resistance profile of a spinach plant to Peronospora farinosa f sp. spinaciae, comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS-LRR protein that comprises in its amino acid sequence: the motif “MAEIGYSVC” at its N-terminus, and the motif “KWMCLR” for alpha-type WOLF proteins or “HVGCVVDR” for beta-type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance to Peronospora farinosa f sp. spinaciae in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races of Peronospora farinosa f. sp. spinaciae in a spinach plant and to primers for use in these methods.
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公开(公告)号：US10577624B2
公开(公告)日：2020-03-03
申请号：US15267351
申请日：2016-09-16
发明人： Cornelis Maria Petrus Van Dun , Sara Movahedi , Japser De Joode , Raoul Jacobus Johannes Maria Frijters , Cornelis Haaring , Eric Cornelis Josephus Bal
摘要： The present invention relates to modified RDR1 gene capable of conferring virus resistance to a plant and/or increasing virus resistance in a plant, which modification results in enhanced expression of the RDR1 gene, and wherein the modification is selected from a modification that increases the mRNA level of the RDR1 gene; a modification that increases the level of the RDR1 protein; and/or a modification that increases the activity of the RDR1 protein, as compared to a non-modified wild-type RDR1 gene. The modification may comprise a modification upstream of the coding sequence of the RDR1 gene, such as a modification of a regulatory element, preferably of a cis-acting regulatory element. The modified regulatory element is for example selected from a transcription factor binding site for a transcriptional repressor, the modification of which leads to reduction or absence of transcriptional repression; and/or a transcription factor binding site for a transcriptional activator, the modification of which leads to induction or enhancement of transcription; and/or a microRNA binding site, the modification of which leads to reduction or absence of gene repression; and/or a small RNA sequence, the modification of which leads to reduction or absence of gene repression.
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公开(公告)号：US20190233841A1
公开(公告)日：2019-08-01
申请号：US16361491
申请日：2019-03-22
发明人： Vincent Laurens Adrianus Kock , Raoul Jacobus Johannes Maria Frijters , Johannes Geert Jan Feitsma
IPC分类号： C12N15/82 , C12Q1/6895 , C12Q1/6806 , C12Q1/686
CPC分类号： C12N15/8282 , A01H5/12 , C07K14/415 , C12Q1/6806 , C12Q1/686 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention relates to an allele designated alpha-WOLF 15 which confers resistance to at least one Peronospora farinosa f. Sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” at its N-terminus; and b) the motif “KWMCLR”; and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele when homozygously present in a spinach plant confers complete resistance to Peronospora farinosa f. Sp. spinacea races pfs:1, pfs:2, pfs:3, pfs:4 and pfs:5, pfs:6, pfs:8, pfs:9, pfs:11, pfs:12, pfs:13, pfs:14, pfs:15, pfs:16 and isolates UA1014 and US1508, and confers intermediate resistance to pfs:10, and does not confer resistance to pfs:7.
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公开(公告)号：US11603538B2
公开(公告)日：2023-03-14
申请号：US16722582
申请日：2019-12-20
IPC分类号： C12N15/82 , A01H5/12 , C12Q1/6895
摘要： The present invention relates to an allele designated alpha-WOLF 25 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” at its N-terminus; and b) the motif “KWMCLR”; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 5. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f sp. spinacea race Pfs:8, Pfs15 and Pfs:16, and does not confer resistance to Pfs:3.
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公开(公告)号：US11434500B2
公开(公告)日：2022-09-06
申请号：US16814006
申请日：2020-03-10
IPC分类号： C12N15/82 , C12N9/12 , C12Q1/6895
摘要： The present invention relates to a genetic determinant which may comprise at least two copies of a combination of two closely linked RDR1 genes, which two closely linked RDR1 genes are inversely oriented, and which genetic determinant leads to virus resistance when present in a plant. In one embodiment, of the RDR1 genes in the combination is represented by SEQ ID NO: 1 or has at least 70% sequence identity, and one of the RDR1 genes in the combination is represented by SEQ ID NO: 3 or has at least 70% sequence identity; or one of the RDR1 genes in the combination encodes a protein represented by SEQ ID NO: 2 or a protein that has at least 70% sequence identity, and one of the RDR1 genes encodes a protein represented by SEQ ID NO: 4 or a protein that has at least 70% sequence identity.
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公开(公告)号：US20220135996A1
公开(公告)日：2022-05-05
申请号：US17085897
申请日：2020-10-30
IPC分类号： C12N15/82
摘要： The present invention relates to an allele designated alpha-WOLF 26 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that may comprise in its amino acid sequence: a) the motif “MAEIGYSVC” SEQ ID NO: 1 at its N-terminus; and b) the motif “KWMCLR” SEQ ID NO: 2; and wherein the LRR domain of the protein has in order of increased preference at least 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5%, 99%, 99.5%, 100% sequence identity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:7, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, Pfs:16, Pfs:17, and does not confer resistance to downy mildew race Pfs:8 and Pfs:9.
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公开(公告)号：US10889829B2
公开(公告)日：2021-01-12
申请号：US15924473
申请日：2018-03-19
IPC分类号： C12N15/82 , C12N9/12 , C12Q1/6895 , A01H5/08
摘要： The present invention relates to a genetic determinant which may comprise at least two copies of a combination of two closely linked RDR1 genes, which two closely linked RDR1 genes are inversely oriented, and which genetic determinant leads to virus resistance when present in a plant. In one embodiment, of the RDR1 genes in the combination is represented by SEQ ID No. 1 or has at least 70% sequence identity, and one of the RDR1 genes in the combination is represented by SEQ ID No. 3 or has at least 70% sequence identity; or one of the RDR1 genes in the combination encodes a protein represented by SEQ ID No. 2 or a protein that has at least 70% sequence identity, and one of the RDR1 genes encodes a protein represented by SEQ ID No. 4 or a protein that has at least 70% sequence identity.
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