公开(公告)号：US20220015638A1

公开(公告)日：2022-01-20

申请号：US17309374

申请日：2019-11-22

Applicant: PROVINCIAL HEALTH SERVICES AUTHORITY

Inventor： Haishan ZENG ,  Zhenguo WU ,  Harvey LUI ,  Yunxian TIAN

IPC: A61B5/00 ,  G02B21/00 ,  G02B21/36 ,  G02B27/64

Abstract: Methods and apparatus for obtaining 3D imaging of tissue involve scanning a focused laser beam in xz planes to obtain a set of xz plane images spaced apart in a y direction. The xz plane images are processed to correct distortions and motions and combined to provide 3D image data. Surface flatting is optionally performed. Imaging may be performed using a femtosecond (fs) laser beam. Different components of light returning from the tissue may be detected and processed to yield plural co-registered images using different imaging modalities, for example, reflective confocal microscopy (RCM), two photon fluorescence (TPF) and second harmonic generation (SHG).