公开(公告)号：US20210060044A1

公开(公告)日：2021-03-04

申请号：US16993447

申请日：2020-08-14

Applicant: POSTECH Research and Business Development Foundation

Inventor： Kunyoo SHIN ,  SungEun KIM ,  Eunjee KIM

IPC: A61K31/706 ,  A61P35/00 ,  C12Q1/6869

Abstract: The present invention relates to a composition for treating bladder cancer, which includes a methylation inhibitor of the Sonic hedgehog (SHH) gene, and more particularly, to a composition for preventing or treating bladder cancer, which includes a methylation inhibitor as an active ingredient to activate a hedgehog (Hh) signaling pathway involving a protein encoded by the gene by maintaining the expression level of SHH gene by suppressing methylation at the specific sites of the promoter of the SHH gene.
The inventors found that the methylation at specific sites of the promoter of the SHH gene changes the pattern of the gene expression, and first identified that bladder cancer can be prevented or treated by controlling the Hh signaling pathway involving a protein encoded by the gene. Therefore, since the growth of cancer cells may be inhibited by inducing differentiation of the bladder cancer cells to a luminal subtype by activating the Hh signaling pathway by suppressing the methylation of the promoter of the SHH gene, the composition according to the present invention is expected to be effectively used in the treatment of bladder cancer.

公开(公告)号：US20210079358A1

公开(公告)日：2021-03-18

申请号：US17005674

申请日：2020-08-28

Applicant: POSTECH Research and Business Development Foundation

Inventor： Kunyoo SHIN ,  Eunjee KIM ,  Sungjune JUNG

IPC: C12N5/071

Abstract: The present invention relates to a 3 dimensional mimetic tissue structure—“Assembloid” based on patient-derived multiple cell types to develop next generation organoid technology serving as a novel platform for new drug development and a disease model and a method of manufacturing the same, and more particularly, to a stem cell- or tumor cell-based 3D multicellular mimetic tissue structure manufactured by reconstituting epithelial or tumor cells with various cellular components of a microenvironment such as stromal cells, vascular cells, immune cells or muscle cells based on three-dimensional (3D) bioprinting, and a method of manufacturing the same. As the stem cell- or tumor cell-based 3D multicellular mimetic tissue structure containing the major factors of a tissue microenvironment, such as stromal cells, vascular cells, immune cells and muscle cells, designed according to the present invention is confirmed to mimic physiological and pathological characteristics of tissue in the body better than conventional organoids, normal and tumor assembloids may be used as a new platform for new drug development and a disease model. More specifically, together with 3D bioprinting technology, it is expected that in vitro bladder tissue and bladder tumor tissue are effectively used as a platform to develop precise and personalized therapeutic options for bladder related diseases including bladder cancer.