公开(公告)号：US10544395B2

公开(公告)日：2020-01-28

申请号：US15126489

申请日：2015-03-17

Applicant: Pfizer Inc.

Inventor： Gregory Walter Hiller ,  Bhanu Chandra Mulukutla

IPC: C12N5/00 ,  C12N5/071 ,  C12P21/02

Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.

公开(公告)号：US10738334B2

公开(公告)日：2020-08-11

申请号：US15762345

申请日：2016-09-22

Applicant: PFIZER INC.

Inventor： Gregory Walter Hiller ,  Jeffrey Joseph Mitchell ,  Bhanu Chandra Mulukutla ,  Pamela Mary Pegman

IPC: C12N15/11 ,  C12P7/62 ,  C12P7/42 ,  C12P7/52 ,  C07K16/00 ,  C12P7/56 ,  C12P7/40 ,  C12P17/18

Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

公开(公告)号：US20200239852A1

公开(公告)日：2020-07-30

申请号：US16722541

申请日：2019-12-20

Applicant: Pfizer Inc.

Inventor： Gregory Walter Hiller ,  Bhanu Chandra Mulukutla

IPC: C12N5/071 ,  C12N5/00

Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.

公开(公告)号：US11718864B2

公开(公告)日：2023-08-08

申请号：US16917099

申请日：2020-06-30

Applicant: PFIZER INC.

Inventor： Gregory Walter Hiller ,  Jeffrey Joseph Mitchell ,  Bhanu Chandra Mulukutla ,  Pamela Mary Pegman

IPC: C12N15/62 ,  C12P7/62 ,  C12P7/42 ,  C12P7/52 ,  C07K16/00 ,  C12P7/56 ,  C12P7/40 ,  C12P17/18

CPC classification number: C12P7/62 ,  C07K16/00 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P7/56 ,  C12P17/182 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2511/00

Abstract: The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.