公开(公告)号：US10092655B2

公开(公告)日：2018-10-09

申请号：US15504239

申请日：2015-08-07

Applicant: NOF CORPORATION ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

Inventor： Shota Sasaki ,  Masaki Ota ,  Kazuhiro Kubo ,  Hideyoshi Harashima ,  Hidetaka Akita ,  Hiroto Hatakeyama ,  Yuki Noguchi ,  Kota Tange ,  Yuta Nakai ,  Nayuta Shimizu

IPC: C12P7/64 ,  A61K9/107 ,  A61K9/127 ,  A61K47/48

Abstract: The invention provides a cationic lipid capable of achieving higher intracellular delivery efficiency than conventional cationic lipids, when used as a lipid membrane structure which is a carrier for delivering functional nucleic acid. The cationic lipid is represented by the formula (1): wherein each symbol is as defined herein.

公开(公告)号：US12018120B2

公开(公告)日：2024-06-25

申请号：US17436738

申请日：2020-03-25

Applicant: NOF CORPORATION

Inventor： Shota Sasaki ,  Yuichi Takahashi ,  Atsushi Sato ,  Tsuyoshi Takehana ,  Toshihiko Hagihara

IPC: C08G65/332

CPC classification number: C08G65/3322

Abstract: A method for producing a terminal carboxyl group-containing polyethylene glycol, which includes the following steps 1 and 2. Step 1: In a solution containing a polyethylene glycol having a hydroxyl group, an inorganic base 0.5 to 4 times the polyethylene glycol in mass, an aprotic solvent and water, mass of the water being 5 to 20 meq with respect to the inorganic base and mass of the water in the solution being 2,000 ppm or less, a compound of the formula (1) is reacted with the polyethylene glycol to obtain a polyethylene glycol ester:




wherein X represents a leaving group, a represents an integer of 4 to 9, and R1 represents a hydrocarbon group having 1 to 4 carbon atoms; Step 2: The polyethylene glycol ester is hydrolyzed to obtain a terminal carboxyl group-containing polyethylene glycol.