公开(公告)号：US20240263210A1

公开(公告)日：2024-08-08

申请号：US18465824

申请日：2023-09-12

Applicant: Nanyang Technological University

Inventor： Bin Wu ,  Renliang Yang ,  Yee Hwa Wong ,  Julien Lescar ,  Chuan Fa Liu ,  James P Tam ,  Kien Truc Giang Nguyen ,  Ziqi Long

IPC: C12P21/02 ,  C07K14/415 ,  C12N9/00

CPC classification number: C12P21/02 ,  C07K14/415 ,  C12N9/93

Abstract: The present invention relates to identification and utilization of a novel structural feature governing efficient peptide ligation of OaAEP1 and a method of forming a peptide of Formula (I) by ligating a first peptide of Formula (II) to a second peptide of Formula (III) by enzymatically cleaving the bond between “Asx” and “Xaa3” in the first peptide of Formula (II) and ligating the fragment P1-Asx of the first peptide via its C-terminus to the N-terminus of the second peptide of Formula (III) to form a ligated peptide of Formula (I), wherein the enzymatic cleavage and ligation reaction is catalyzed by a peptide ligase having the activity of OaAEP1 Cys247Ala (SEQ ID NO:1) under conditions suitable for said cleavage and ligation reaction. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest catalyzed by the peptide ligase having the activity of OaAEP1 Cys247Ala (SEQ ID NO:1). Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods of the invention, the peptide ligase OaAEP1 Cys247Ala having the amino acid sequence of SEQ ID NO:1 and other peptide ligases having the activity thereof, as well as kits comprising any of said peptide ligases, in particular OaAEP1 Cys247Ala (SEQ ID NO:1).

公开(公告)号：US11795488B2

公开(公告)日：2023-10-24

申请号：US16336023

申请日：2017-09-12

Applicant: NANYANG TECHNOLOGICAL UNIVERSITY

Inventor： Bin Wu ,  Renliang Yang ,  Yee Hwa Wong ,  Julien Lescar ,  Chuan Fa Liu ,  James P Tam ,  Kien Truc Giang Nguyen ,  Ziqi Long

IPC: C12P21/02 ,  C12N9/00 ,  C07K14/415

CPC classification number: C12P21/02 ,  C07K14/415 ,  C12N9/93

Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.