公开(公告)号：US20100190259A1

公开(公告)日：2010-07-29

申请号：US12391366

申请日：2009-02-24

Applicant: Muhammed JAVED ,  Fiona CUSDIN ,  Paul MILNER ,  Edward GREEN

Inventor： Muhammed JAVED ,  Fiona CUSDIN ,  Paul MILNER ,  Edward GREEN

IPC: C12N15/74

CPC classification number: C12N9/00 ,  C12N9/0004 ,  C12P7/065 ,  Y02E50/17

Abstract: The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination. The method is particularly useful in connection with species of Bacillus such as B. stereothermophilus, B. calvodelox, B. caldotenax, B. thermoglucosidasius, B. coagulans, B. licheniformis, B. thermodenitrificans, and B. caldolyticus.

Abstract translation: 本发明涉及作为细菌发酵产物的乙醇的生产。 特别地，本发明涉及基于同源重组的基因失活和基因表达的新方法。 该方法特别适用于芽孢杆菌属物种，例如立体嗜热嗜热芽孢杆菌，卡尔伐氏酵母，卡氏杆菌，热霉葡萄糖杆菌，凝结芽孢杆菌，地衣芽孢杆菌，嗜热嗜热芽孢杆菌和热卡氏弧菌。