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公开(公告)号：US20240342263A1
公开(公告)日：2024-10-17
申请号：US18757757
申请日：2024-06-28
Applicant: Merck Sharp & Dohme LLC
Inventor： Jitin Bajaj , Michael Albert Winters , Emily Wen , Jian He
IPC: A61K39/09 , A61K39/385 , A61K39/00
CPC classification number: A61K39/092 , A61K39/385 , A61K2039/6037 , A61K2039/62
Abstract: The present invention provides methods for making polysaccharide-protein conjugates in which polysaccharides, typically from bacteria, are conjugated to a carrier protein by reductive amination under conditions which improve conjugation reaction consistency, increase consumption of protein during conjugation reaction, generate conjugates of higher molecular weight, and/or reduce the levels of free cyanide in the conjugate reaction product. The polysaccharide-protein conjugates obtained using these methods are useful for inclusion in multivalent vaccines.
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公开(公告)号：US12144855B2
公开(公告)日：2024-11-19
申请号：US17049208
申请日：2019-04-25
Applicant: Merck Sharp & Dohme LLC
Inventor： Patrick Leonard Ahl , Akhilesh Bhambhani , Christopher Jon Farrell , Patrick McHugh , Morrisa C. Jones , Daniel D. Roth , Jessica R. Sinacola , Justin Stanbro , Matthew P. Watson , Emily Wen , Michael A. Winters
IPC: A61K39/02 , A61K9/19 , A61K39/09 , A61K39/116 , A61K47/61 , A61K47/64 , A61K47/68 , A61K47/69 , A61K39/00
Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized in the form of lyospheres of carrier protein and lyospheres of the one or more polysaccharides. A predetermined amount of carrier protein lyospheres and activated polysaccharide lyospheres are mixed together and the mixture reconstituted in an organic solvent to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.
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Patent Agency Ranking
3.发明公开公开(公告)号：US20240261384A1
公开(公告)日：2024-08-08
申请号：US18637589
申请日：2024-04-17
Applicant: Merck Sharp & Dohme LLC
Inventor： Patrick Leonard Ahl , Akhilesh Bhambhani , Christopher Jon Farrell , Patrick McHugh , Morrisa C. Jones , Daniel D. Roth , Jessica R. Sinacola , Justin Stanbro , Matthew P. Watson , Emily Wen , Michael A. Winters
CPC classification number: A61K39/092 , A61K47/61 , A61K47/62 , A61K2039/6037 , A61K2039/64 , A61K2039/70
Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.
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公开(公告)号：US11992521B2
公开(公告)日：2024-05-28
申请号：US18049507
申请日：2022-10-25
Applicant: Merck Sharp & Dohme LLC
Inventor： Patrick Leonard Ahl , Akhilesh Bhambhani , Christopher Jon Farrell , Patrick McHugh , Morrisa C. Jones , Daniel D. Roth , Jessica R. Sinacola , Justin Stanbro , Matthew P. Watson , Emily Wen , Michael A. Winters
CPC classification number: A61K39/092 , A61K47/61 , A61K47/62 , A61K2039/6037 , A61K2039/64 , A61K2039/70
Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.
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公开(公告)号：US11896656B2
公开(公告)日：2024-02-13
申请号：US17049266
申请日：2019-04-25
Applicant: Merck Sharp & Dohme LLC
Inventor： Patrick Leonard Ahl , Akhilesh Bhambhani , Christopher Jon Farrell , Patrick McHugh , Morrisa C. Jones , Daniel D. Roth , Jessica R. Sinacola , Justin Stanbro , Matthew P. Watson , Emily Wen , Michael A. Winters
CPC classification number: A61K39/092 , A61K39/39 , A61K2039/6037
Abstract: A method is described for reconstituting lyophilized mutant diphtheria toxin in dimethyl sulfoxide for use in producing pneumococcal capsular polysaccharide mutant diphtheria toxin conjugates.
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公开(公告)号：US12059461B2
公开(公告)日：2024-08-13
申请号：US17454336
申请日：2021-11-10
Applicant: Merck Sharp & Dohme LLC
Inventor： Jitin Bajaj , Michael Albert Winters , Emily Wen , Jian He
IPC: A61K39/09 , A61K39/00 , A61K39/385
CPC classification number: A61K39/092 , A61K39/385 , A61K2039/6037 , A61K2039/62
Abstract: The present invention provides methods for making polysaccharide-protein conjugates in which polysaccharides, typically from bacteria, are conjugated to a carrier protein by reductive amination under conditions which improve conjugation reaction consistency, increase consumption of protein during conjugation reaction, generate conjugates of higher molecular weight, and/or reduce the levels of free cyanide in the conjugate reaction product. The polysaccharide-protein conjugates obtained using these methods are useful for inclusion in multivalent vaccines.
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7.发明申请公开(公告)号：US20230077640A1
公开(公告)日：2023-03-16
申请号：US18049507
申请日：2022-10-25
Applicant: Merck Sharp & Dohme LLC
Inventor： Patrick Leonard Ahl , Akhilesh Bhambhani , Christopher Jon Farrell , Patrick McHugh , Morrisa C. Jones , Daniel D. Roth , Jessica R. Sinacola , Justin Stanbro , Matthew P. Watson , Emily Wen , Michael A. Winters
Abstract: A method is described for producing a pneumococcal capsular polysaccharide protein conjugate in which one or more activated pneumococcal polysaccharides of particular pneumococcal serotypes and carrier protein are separately lyophilized, the separately lyophilized polysaccharides and carrier protein are separately reconstituted in an organic solvent, and the reconstituted polysaccharide and carrier protein are then combined together by Tee-mixing and conjugated together to produce polysaccharide carrier protein conjugates. A plurality of conjugates, each comprising polysaccharides of a particular serotype, may be used to produce multivalent pneumococcal immunogenic compositions having a combination of conjugates for use in vaccines.
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