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公开(公告)号:US20110039292A1
公开(公告)日:2011-02-17
申请号:US12539153
申请日:2009-08-11
IPC分类号: C12Q1/02
CPC分类号: G01N33/5014 , G01N2333/4709 , G01N2510/00
摘要: Disclosed is a bioassay system in an in-vitro test that enables higher quality guarantee of yokukansan. A bioassay for yokukansan comprises the steps of culturing cells in a culture medium with serum, adding yokukansan to the cells in a serum-free medium, applying ER stress to the cells to induce cell death, and determining a pharmacological activity value of yokukansan from cell viability in the cells.
摘要翻译: 公开了一种体外测试中的生物测定系统,其能够提供高质量的洋葱素保证。 对于洋葱素的生物测定包括以下步骤:在培养基中培养血清,向无血清培养基中的细胞中加入洋甘草素,向细胞施加ER应激以诱导细胞死亡,并从细胞中测定洋葱素的药理学活性值 细胞的活力。