公开(公告)号：US20200208126A1

公开(公告)日：2020-07-02

申请号：US16633942

申请日：2017-08-09

申请人： MGI Tech Co., Ltd.

发明人： Zhougang Zhang ,  Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xing Liu ,  Yuliang Dong ,  Chongjun Xu ,  Wenwei Zhang

IPC分类号： C12N9/12

摘要： Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

公开(公告)号：US11104889B2

公开(公告)日：2021-08-31

申请号：US16633942

申请日：2017-08-09

申请人： MGI Tech Co., Ltd.

发明人： Zhougang Zhang ,  Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xing Liu ,  Yuliang Dong ,  Chongjun Xu ,  Wenwei Zhang

IPC分类号： C12N9/12

摘要： Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

公开(公告)号：US10961519B2

公开(公告)日：2021-03-30

申请号：US16657713

申请日：2019-10-18

申请人： MGI TECH CO., LTD.

发明人： Huanhuan Liu ,  Yue Zheng ,  Yujun Zhou ,  Xi Zhang ,  Zhougang Zhang ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu ,  Snezana Drmanac

IPC分类号： C12N9/12 ,  C12N7/00

摘要： Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.