公开(公告)号：US20160000896A1

公开(公告)日：2016-01-07

申请号：US14851457

申请日：2015-09-11

Applicant: Merial Inc.

Inventor： Nicolas Pierre Yves Carboulec ,  Rene Labatut ,  Lionel Gerentes

IPC: A61K39/02

CPC classification number: A61K39/099 ,  A61K39/0225 ,  A61K39/0241 ,  A61K2039/521 ,  A61K2039/552

Abstract: The invention relates to hyperbaric devices for inactivating microorganisms and viruses while retaining their immunogenicity and for making and producing the soluble, disaggregated, refolded or active immunogenic or therapeutic proteins from inclusion bodies produced from prokaryotes or eukaryotes. The invention encompasses hyperbaric methods for inactivating pathogenic organisms, and methods for producing vaccine compositions using the inactivated pathogens. The hyperbarically inactivated microorganisms are safer and more immunogenic than chemically inactivated microorganisms. Similarly, the solubilized proteins have superior properties compared to more heavily aggregated proteins, including reduced non-specific immune reactions.

Abstract translation: 本发明涉及用于灭活微生物和病毒的高压装置，同时保留其免疫原性并用于制备和产生可从原核生物或真核生物产生的包涵体的可溶性，分解，重折叠或活性免疫原性或治疗性蛋白质。 本发明包括用于灭活致病生物体的高压方法，以及使用灭活病原体生产疫苗组合物的方法。 高温灭活的微生物比化学灭活的微生物更安全，更具免疫原性。 类似地，与更重聚集的蛋白质相比，溶解的蛋白质具有优异的性质，包括减少的非特异性免疫反应。

公开(公告)号：US20170081372A1

公开(公告)日：2017-03-23

申请号：US15017486

申请日：2016-02-05

Applicant: MERIAL INC.

Inventor： Laurent Bernard Fischer ,  Nicolas Pierre Yves Carboulec ,  Fabien Lux

IPC: C07K14/44 ,  C12N9/50 ,  A61K39/008 ,  C12N9/10

CPC classification number: C07K14/245 ,  A23L3/0155 ,  A61K39/008 ,  A61K39/0225 ,  A61K39/0241 ,  A61K39/099 ,  A61K2039/552 ,  A61L2/0011 ,  C07K14/44 ,  C07K2319/00 ,  C07K2319/35 ,  C07K2319/40 ,  C12N5/0031 ,  C12N9/1007 ,  C12N9/50 ,  C12N9/52 ,  C12P21/02 ,  C12Y201/01041 ,  C12Y304/22

Abstract: The present invention encompasses vaccines or compositions comprising the chimeric KSAC protein that possesses immunogenic and protective properties, and methods of use including administering to an animal the antigenic KSAC protein thereof to protect animals. The invention also encompasses methods for making and producing the soluble, disaggregated, refolded or active proteins from inclusion bodies produced from prokaryotes or eukaryotes.

公开(公告)号：US10080791B2

公开(公告)日：2018-09-25

申请号：US14851457

申请日：2015-09-11

Applicant: Merial Inc.

Inventor： Nicolas Pierre Yves Carboulec ,  Rene Labatut ,  Lionel Gerentes

IPC: A61L2/00 ,  A61K39/02 ,  A61K39/00

CPC classification number: A61K39/099 ,  A61K39/0225 ,  A61K39/0241 ,  A61K2039/521 ,  A61K2039/552

Abstract: The invention relates to hyperbaric devices for inactivating microorganisms and viruses while retaining their immunogenicity and for making and producing the soluble, disaggregated, refolded or active immunogenic or therapeutic proteins from inclusion bodies produced from prokaryotes or eukaryotes. The invention encompasses hyperbaric methods for inactivating pathogenic organisms, and methods for producing vaccine compositions using the inactivated pathogens. The hyperbarically inactivated microorganisms are safer and more immunogenic than chemically inactivated microorganisms. Similarly, the solubilized proteins have superior properties compared to more heavily aggregated proteins, including reduced non-specific immune reactions.