公开(公告)号：US20190170750A1

公开(公告)日：2019-06-06

申请号：US16307710

申请日：2017-06-02

Applicant: MEDIZINISCHE UNIVERSITÄT WIEN

Inventor： Franz X. HEINZ ,  Karin STIASNY

IPC: G01N33/569

Abstract: Disclosed is a method for the detection of an IgM antibody specific for a flavivirus in a sample, comprising the steps of (a) contacting the sample with a solid support comprising immobilised IgM-binding molecules, (b) allowing binding of IgM antibodies in the sample to the IgM binding molecules on the solid support so that the IgM antibodies are also immobilised on the solid support, and (c) detecting IgM antibodies specific for a flavivirus by allowing binding of a complex comprising (i) an antiparallel dimer of soluble flavivirus Protein E (sE) and (ii) a marker and identifying the binding of the complex to the specific flavivirus IgM antibody by detecting the marker; and a kit suitable for performing the method.