公开(公告)号：US20090263902A1

公开(公告)日：2009-10-22

申请号：US12357284

申请日：2009-01-21

申请人： Luke MEHLO ,  Zuo-Yu Zhao

发明人： Luke MEHLO ,  Zuo-Yu Zhao

IPC分类号： C12N15/82 ,  C12N1/20

CPC分类号： C12N15/8205

摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.

摘要翻译： 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。