公开(公告)号：US20190079095A1

公开(公告)日：2019-03-14

申请号：US16107762

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: G01N33/58 ,  G01N33/533 ,  C07D213/68 ,  C07D213/53 ,  C07D235/14 ,  C07D401/04 ,  C07D215/12 ,  C07D213/79 ,  C07D213/74 ,  C07K14/575 ,  C07D401/14 ,  C07D213/78 ,  C07D213/81

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US10648985B2

公开(公告)日：2020-05-12

申请号：US16107762

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: C07D213/46 ,  G01N33/58 ,  G01N33/533 ,  C07D213/74 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US11067580B2

公开(公告)日：2021-07-20

申请号：US16838004

申请日：2020-04-01

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: C07D213/74 ,  G01N33/58 ,  G01N33/533 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US20200333351A1

公开(公告)日：2020-10-22

申请号：US16838004

申请日：2020-04-01

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Upinder Singh ,  Scott Grecian ,  Scott Clarke

IPC: G01N33/58 ,  G01N33/533 ,  C07D213/74 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D401/04 ,  C07D235/14 ,  C07D213/53 ,  C07D213/68 ,  C07D213/78 ,  C09B23/06 ,  C09B23/08 ,  C09B11/24 ,  C07D401/14 ,  C07K14/575

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：US10138510B2

公开(公告)日：2018-11-27

申请号：US14300728

申请日：2014-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Clarke ,  Jolene Bradford

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6804

Abstract: The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.