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    Anti-Viral Azide Containing Compounds
    3.
    发明申请
    Anti-Viral Azide Containing Compounds 有权
    抗病毒叠氮化合物

    公开(公告)号：US20150374723A1

    公开(公告)日：2015-12-31

    申请号：US14831220

    申请日：2015-08-20

    Applicant: Life Technologies Corporation ,  The Johns Hopkins University

    Inventor： Brian Agnew ,  David Graham ,  Upinder Singh ,  Scott Grecian

    IPC: A61K31/655 ,  A61K38/21 ,  A61K31/7088 ,  A61K45/06

    CPC classification number: A61K31/655 ,  A61K31/7024 ,  A61K31/7088 ,  A61K38/212 ,  A61K38/215 ,  A61K45/06 ,  A61K2300/00

    Abstract: Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

    Abstract translation: 提供了使用叠氮化物修饰的生物分子如脂肪酸，碳水化合物和脂质来治疗植物，昆虫或感染病毒的动物或抑制病毒如人类免疫缺陷病毒的感染性的方法。 还提供了用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质来标记病毒（例如人类免疫缺陷病毒）的方法。 此外，提供了利用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质体内追踪病毒的方法。 叠氮化物修饰的生物分子可以与药学上可接受的赋形剂组合以产生药物组合物，任选地含有另一种抗病毒剂和/或递送剂，例如脂质体。

    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS
    6.
    发明申请
    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20170137866A1

    公开(公告)日：2017-05-18

    申请号：US15332304

    申请日：2016-10-24

    Applicant: President and Fellows of Harvard College ,  Life Technologies Corporation

    Inventor： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC: C12Q1/68

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES
    7.
    发明申请
    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES 审中-公开
    增加病毒感染的方法

    公开(公告)号：US20150017703A1

    公开(公告)日：2015-01-15

    申请号：US14374345

    申请日：2013-01-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian Agnew

    IPC: C12N7/00

    CPC classification number: C12N7/00 ,  A61K39/00 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2810/10

    Abstract: Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

    Abstract translation: 使用用炔烃修饰的生物分子（例如脂肪酸，碳水化合物和脂质）标记的病毒的方法来治疗感染病毒的植物，昆虫或动物或增加病毒如人免疫缺陷病毒的感染性， 被提供。 还提供了用炔修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质来标记病毒（例如人类免疫缺陷病毒）的方法。 用炔烃修饰的生物分子标记的病毒可以与药学上可接受的赋形剂组合以产生药物组合物，任选地含有另一种抗病毒剂和/或递送剂，例如脂质体。

    Methods and compositions for labeling nucleic acids
    8.
    发明授权
    Methods and compositions for labeling nucleic acids 有权

    公开(公告)号：US10550422B2

    公开(公告)日：2020-02-04

    申请号：US15332304

    申请日：2016-10-24

    Applicant: President and Fellows of Harvard College ,  Life Technologies Corporation

    Inventor： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC: C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/50 ,  G01N33/58

    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES
    9.
    发明申请
    METHODS FOR INCREASING THE INFECTIVITY OF VIRUSES 审中-公开

    公开(公告)号：US20180201907A1

    公开(公告)日：2018-07-19

    申请号：US15920191

    申请日：2018-03-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian Agnew

    IPC: C12N7/00

    CPC classification number: C12N7/00 ,  A61K39/00 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2810/10

    Abstract: Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The viruses labeled with alkyne-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

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