公开(公告)号：US06355440B1

公开(公告)日：2002-03-12

申请号：US08459082

申请日：1995-06-02

Applicant: Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

Inventor： Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

IPC: G01N3353

CPC classification number: C07K14/71 ,  A61K38/00 ,  G01N33/74 ,  G01N2333/50 ,  G01N2333/71 ,  G01N2500/00

Abstract: The present invention relates to receptors for growth factors, specifically to the fibroblast growth factor receptor (FGF-R). More particularly, it provides various purified fibroblast growth factor receptor proteins, nucleic acids encoding the receptor proteins, methods for the production of purified FGF-R proteins, proteins made by these methods, antibodies against these proteins, diagnostic and therapeutic uses of these various reagents, and methods of detecting a fibroblast growth factor in a sample using the disclosed fibroblast growth factor receptors.

Abstract translation: 本发明涉及生长因子受体，特别涉及成纤维细胞生长因子受体（FGF-R）。 更具体地，其提供各种纯化的成纤维细胞生长因子受体蛋白质，编码受体蛋白质的核酸，用于产生纯化的FGF-R蛋白质的方法，通过这些方法制备的蛋白质，针对这些蛋白质的抗体，这些各种试剂的诊断和治疗用途 以及使用所公开的成纤维细胞生长因子受体检测样品中成纤维细胞生长因子的方法。

公开(公告)号：US06350593B1

公开(公告)日：2002-02-26

申请号：US08459395

申请日：1995-06-02

Applicant: Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

Inventor： Lewis T. Williams ,  Daniel F. Johnson ,  Pauline E Lee

IPC: C12N1509

CPC classification number: C07K14/71 ,  A61K38/00 ,  G01N33/74 ,  G01N2333/50 ,  G01N2333/71 ,  G01N2500/00

Abstract: A fibroblast growth factor (FGF) receptor including a basic fibroblast growth factor receptor has been purified. Various forms have bee identified including soluble forms lacking any transmembrane segment. DNA sequences encoding full-length fibroblast growth factor receptors and polypeptides comprising a portion of an FGF-R ligand-binding domain have been isolated and sequenced. These DNAs include DNAs encoding for a basic FGF-R and a human FGF-R and are operably linked to control sequences and expressed in a culture of a compatible host transformed, transfected or electroporated by a cloning vehicle containing the DNA sequence. The invention also comprises antibodies to the receptor, methods of synthesizing the growth factor receptor proteins, methods for providing analogs of the fibroblast growth factor receptors. Methods for evaluating compositions which promoter or inhibit fibroblastic growth factors and compositions which are agonistic or antagonistic to fibroblast growth factor receptors are also provided. Diagnostic and therapeutic uses are described.

Abstract translation: 已经纯化了包含碱性成纤维细胞生长因子受体的成纤维细胞生长因子（FGF）受体。 鉴别出各种形式的蜜蜂，包括没有任何跨膜片段的可溶形式。 已经分离并测序了编码全长成纤维细胞生长因子受体的DNA序列和包含一部分FGF-R配体结合结构域的多肽。 这些DNA包括编码碱性FGF-R和人FGF-R的DNA，并且与对照序列可操作地连接，并在通过含有DNA序列的克隆载体转化，转染或电穿孔的相容宿主的培养物中表达。 本发明还包括对受体的抗体，合成生长因子受体蛋白质的方法，提供成纤维细胞生长因子受体类似物的方法。 还提供了用于评估启动子或抑制成纤维细胞生长因子和对成纤维细胞生长因子受体具有激动作用或拮抗作用的组合物的组合物的方法。 描述诊断和治疗用途。

公开(公告)号：US06384191B1

公开(公告)日：2002-05-07

申请号：US07834311

申请日：1992-02-13

Applicant: Lewis T. Williams ,  Daniel E. Johnson ,  Pauline E Lee

Inventor： Lewis T. Williams ,  Daniel E. Johnson ,  Pauline E Lee

IPC: C07K1471

CPC classification number: C07K14/71 ,  A61K38/00 ,  G01N33/74 ,  G01N2333/50 ,  G01N2333/71 ,  G01N2500/00

Abstract: A fibroblast growth factor (FGF) receptor including a basic fibroblast growth factor receptor has been purified. Various forms have been identified including soluble forms lacking any transmembrane segment. DNA sequences encoding full-length fibroblast growth factor receptors and polypeptides comprising a portion of an FGF-R ligand-binding domain have been isolated and sequenced. These DNAs include DNAs encoding for a basic FGF-R and a human FGF-R and are operably linked to control sequences and expressed in a culture of a compatible host transformed, transfected or electroporated by a cloning vehicle containing the DNA sequence. The invention also comprises antibodies to the receptor, methods of synthesizing the growth factor receptor proteins, methods for providing analogs of the fibroblast growth factor receptors. Methods for evaluating compositions which promote or inhibit fibroblastic growth factors and compositions which are agonistic or antagonistic to fibroblast growth factor receptors are also provided. Diagnostic and therapeutic uses are described.

Abstract translation: 已经纯化了包含碱性成纤维细胞生长因子受体的成纤维细胞生长因子（FGF）受体。 已经鉴定了各种形式，包括不含任何跨膜片段的可溶形式。 已经分离并测序了编码全长成纤维细胞生长因子受体的DNA序列和包含一部分FGF-R配体结合结构域的多肽。 这些DNA包括编码碱性FGF-R和人FGF-R的DNA，并且与对照序列可操作地连接，并在通过含有DNA序列的克隆载体转化，转染或电穿孔的相容宿主的培养物中表达。 本发明还包括对受体的抗体，合成生长因子受体蛋白质的方法，提供成纤维细胞生长因子受体类似物的方法。 还提供了评估促进或抑制成纤维细胞生长因子的组合物和对成纤维细胞生长因子受体具有激动作用或拮抗作用的组合物的方法。 描述诊断和治疗用途。