公开(公告)号：US20230193379A1

公开(公告)日：2023-06-22

申请号：US17811192

申请日：2022-07-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vadim Mozhayskiy ,  Yutao FU ,  Earl HUBBELL

IPC: C12Q1/6874 ,  C12Q1/6825 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12N15/1058

Abstract: A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.