公开(公告)号：US11466312B2

公开(公告)日：2022-10-11

申请号：US16958683

申请日：2018-12-31

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  BIONANO HEALTH GUARD RESEARCH CENTER

Inventor： Hyun Gyu Park ,  Hyo Yong Kim ,  Yong Ju ,  Jun Ki Ahn

IPC: C12Q1/683

Abstract: The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.

公开(公告)号：US20180319835A1

公开(公告)日：2018-11-08

申请号：US15924242

申请日：2018-03-18

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  Gachon University of Industry-Academic cooperation Foundation

Inventor： Hyun Gyu Park ,  Moon Il Kim ,  Ki Soo Park ,  Chang Yeol Lee

IPC: C07H23/00 ,  C07H1/00

CPC classification number: C07H23/00 ,  C07H1/00

Abstract: A method of preparing nucleic acid-inorganic hybrid nanoflowers is described, in which a nucleic acid is allowed to react with a solution of a metal ion-containing compound at room temperature, thereby forming a complex between the metal ion and the nitrogen atom of an amide bond or amine group present in the nucleic acid. Organic-inorganic hybrid nanoflower structures thus may be synthesized using nucleic acid in a simple manner under an environmentally friendly condition without any toxic chemical substance. The produced organic-inorganic hybrid nanoflower structures exhibit a high DNA encapsulation yield, nuclease resistance, and significantly increased peroxidase activity. These nanoflower structures may be widely used as gene therapy carriers and in biosensing technology.

公开(公告)号：US10041111B2

公开(公告)日：2018-08-07

申请号：US15524093

申请日：2015-11-04

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Hyun Gyu Park ,  Ki Soo Park ,  Chang Yeol Lee

IPC: C12Q1/68 ,  C12N15/115 ,  C12Q1/6851

Abstract: The present invention relates to a method of detecting and quantifying biomolecules using nucleic acid polymerase activity controlled by the target molecule, and more particularly to a method for detecting or quantifying biomolecules, which can detect and quantify nucleic acids, proteins, small-molecular substances, physiologically active substances (enzymatic activities), etc., with high sensitivity, based on the change in DNA polymerase activity caused by specific binding of a specific nucleic acid that forms a complex with a DNA aptamer prepared so as to comprise a single-stranded DNA that specifically recognizes the specific nucleic acid. The present invention can provide a method for diagnosing biomolecules, which can detect and quantify target nucleic acids, target proteins, target small-molecular substances, target enzyme activities and the like in a label-free and sensitive manner by controlling polymerase activity through target molecule-induced conformational change of a DNA aptamer.

公开(公告)号：US20170335381A1

公开(公告)日：2017-11-23

申请号：US15524093

申请日：2015-11-04

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Hyun Gyu Park ,  Ki Soo Park ,  Chang Yeol Lee

IPC: C12Q1/68 ,  C12N15/115

CPC classification number: C12Q1/6851 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6804 ,  C12Q1/6848 ,  C12Q2525/101 ,  C12Q2537/163 ,  C12Q2561/101 ,  C12Q2521/514 ,  C12Q2521/531 ,  C12Q2521/301

Abstract: The present invention relates to a method of detecting and quantifying biomolecules using nucleic acid polymerase activity controlled by the target molecule, and more particularly to a method for detecting or quantifying biomolecules, which can detect and quantify nucleic acids, proteins, small-molecular substances, physiologically active substances (enzymatic activities), etc., with high sensitivity, based on the change in DNA polymerase activity caused by specific binding of a specific nucleic acid that forms a complex with a DNA aptamer prepared so as to comprise a single-stranded DNA that specifically recognizes the specific nucleic acid. The present invention can provide a method for diagnosing biomolecules, which can detect and quantify target nucleic acids, target proteins, target small-molecular substances, target enzyme activities and the like in a label-free and sensitive manner by controlling polymerase activity through target molecule-induced conformational change of a DNA aptamer.