公开(公告)号：US20220243186A1

公开(公告)日：2022-08-04

申请号：US17544429

申请日：2021-12-07

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Mihue JANG ,  Junghee KIM ,  Sang Chul SHIN ,  Young Eun LEE ,  Han-Na YOON

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N15/63

Abstract: Provided are a fusion protein used in a CRISPR/Cas system, and a complex including the same and use thereof. Since the gene editing complex including the fusion protein of the present disclosure includes an NKG2D ligand capable of binding to NKG2D expressed on the membrane of natural killer cells, it may be specifically delivered to NKG2D receptor-expressing cells or natural killer cells. Since it may be effectively delivered into the cells through endocytosis of NKG2D without a carrier, a target gene or a target DNA of NKG2D receptor-expressing cells or natural killer cells may be manipulated using the complex.