公开(公告)号：US20180284127A1

公开(公告)日：2018-10-04

申请号：US15945890

申请日：2018-04-05

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： TAE-YOUNG YOON

IPC: G01N33/68 ,  G01N21/64 ,  G01N21/77 ,  G01N33/574

CPC classification number: G01N33/6845 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/77 ,  G01N33/574 ,  G01N2021/7786

Abstract: A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values.

公开(公告)号：US20160327566A1

公开(公告)日：2016-11-10

申请号：US15211096

申请日：2016-07-15

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： TAE-YOUNG YOON

IPC: G01N33/68 ,  G01N21/64

CPC classification number: G01N33/6845 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/77 ,  G01N33/574 ,  G01N2021/7786

Abstract: A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values.

Abstract translation: 分析蛋白质 - 蛋白质相互作用的方法包括将第一蛋白质与底物结合，其中第一蛋白质与第一标记物结合，第一标记物特异性结合固定在底物上的生物分子，或者第一种蛋白质特异性结合固定在底物上的生物分子; 将底物结合的第一蛋白质与含有用第二标记物标记的第二蛋白质的细胞裂解物孵育; 分析细胞裂解物中第一种蛋白质和第二种蛋白质之间的相互作用，并获得表示相互作用动力学图像的第一个分析值; 将底物结合的第一蛋白质与由第二标记标记的第二蛋白质组成的细胞裂解物和包含其他蛋白质的另一细胞裂解物（包括未标记的第二蛋白质）的细胞裂解物混合物孵育并获得第二分析值; 比较和分析第一和第二个分析值。

公开(公告)号：US20170299603A1

公开(公告)日：2017-10-19

申请号：US15642427

申请日：2017-07-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： TAE-YOUNG YOON

IPC: G01N33/68 ,  G01N21/64 ,  G01N33/574 ,  G01N21/77

CPC classification number: G01N33/6845 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/77 ,  G01N33/574 ,  G01N2021/7786

Abstract: A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values.