公开(公告)号：US11655462B2

公开(公告)日：2023-05-23

申请号：US16490515

申请日：2019-05-02

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Kyung-Jin Kim ,  Jung Ho Ahn ,  Hogyun Seo ,  Jong An Lee

IPC: C12N1/20 ,  C12N9/04 ,  C12N15/74 ,  C12P7/46

CPC classification number: C12N9/0006 ,  C12N1/20 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01037

Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.

公开(公告)号：US11542483B2

公开(公告)日：2023-01-03

申请号：US17366269

申请日：2021-07-02

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Junho Bang ,  Chang Hun Hwang ,  Jung Ho Ahn ,  Jong An Lee

IPC: C12P7/46 ,  C12N9/10 ,  C12P7/02 ,  C12N9/04 ,  C12N9/00 ,  C12N9/78 ,  C12P7/16 ,  C12P7/04 ,  C12P13/10 ,  C12P5/00 ,  C12P5/02 ,  C12P13/00 ,  C12P7/42 ,  C12P17/10 ,  C12P13/06 ,  C12P13/08

Abstract: Disclosed is a recombinant microorganism capable of growing using only carbon dioxide and formic acid by introducing and improving a metabolic pathway for synthesizing pyruvic acid from carbon dioxide and formic acid to enhance pyruvic acid synthesis efficiency and performing additional genetic manipulation, and a method for producing useful substances using the same. Advantageously, the recombinant microorganism is capable of synthesizing pyruvic acid, a C3 organic compound, at a remarkably improved rate, and in particular, grows well even in a medium containing only carbon dioxide and formic acid as carbon sources without a glucose supply, and is thereby capable of synthesizing pyruvic acid and various high value-added compounds using the same as an intermediate product in an economically efficient manner.