公开(公告)号：US10227586B2

公开(公告)日：2019-03-12

申请号：US15491154

申请日：2017-04-19

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Younghoon Lee ,  Won Kyong Kim ,  Geunu Bak ,  Hongmarn Park ,  Jee Soo Choi ,  Shinae Suk ,  Ji Young Lee ,  Yeongseong Yoon

IPC: C12N15/10

Abstract: The present invention relates to an artificial ncRNA expression library and a method for preparing the same, and particularly the present invention relates to a library securing the stability of the artificial ncRNA by cloning random fragmented DNA that whole genome DNA of E. coli is randomly fragmented to a middle of RNA scaffold having a double stem-loop and a method for preparing the same.

公开(公告)号：US09834768B2

公开(公告)日：2017-12-05

申请号：US14855612

申请日：2015-09-16

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Younghoon Lee ,  Geunu Bak ,  Hongmarn Park ,  Jee Soo Choi ,  Won Kyeong Kim ,  Shinae Suk ,  Ji Young Lee ,  Yeongseong Yoon

IPC: C07H21/02 ,  G01N33/554 ,  C12N15/113

CPC classification number: C12N15/113 ,  C12N2310/11 ,  C12N2310/531 ,  C12N2330/51

Abstract: A nucleic acid molecule comprising a stem-loop structure, a nucleic acid complex comprising the nucleic acid molecule, a composition for delivering a target recognition sequence, and the composition comprising the nucleic acid complex. An artificial small ribonucleic acid of the stem-loop structure stably maintains single-strandedness of a target recognition sequence which interacts with a nucleic acid of interest for a gene of interest of a prokaryote, thereby providing a nucleic acid complex for effective silencing of the gene of interest.

公开(公告)号：US20160186169A1

公开(公告)日：2016-06-30

申请号：US14855612

申请日：2015-09-16

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Younghoon LEE ,  Geunu Bak ,  Hongmarn Park ,  Jee Soo Choi ,  Won Kyeong Kim ,  Shinae Suk ,  Ji Young Lee ,  Yeongseong Yoon

IPC: C12N15/113 ,  C12N15/70

CPC classification number: C12N15/113 ,  C12N2310/11 ,  C12N2310/531 ,  C12N2330/51

Abstract: A nucleic acid molecule comprising a stem-loop structure, a nucleic acid complex comprising the nucleic acid molecule, a composition for delivering a target recognition sequence, and the composition comprising the nucleic acid complex. An artificial small ribonucleic acid of the stem-loop structure stably maintains single-strandedness of a target recognition sequence which interacts with a nucleic acid of interest for a gene of interest of a prokaryote, thereby providing a nucleic acid complex for effective silencing of the gene of interest.

Abstract translation: 包含茎 - 环结构的核酸分子，包含核酸分子的核酸复合物，用于递送靶识别序列的组合物和包含核酸复合物的组合物。 茎 - 环结构的人造小核糖核酸稳定地维持目标识别序列的单链，其与原核生物感兴趣的基因的目的核酸相互作用，从而提供用于有效沉默基因的核酸复合物 出于兴趣。