公开(公告)号：US20240059756A1

公开(公告)日：2024-02-22

申请号：US18267990

申请日：2021-03-11

申请人： José Adán ORTEGA BLANCO

发明人： Juan Pablo AGUILAR ALEMÁN ,  Beni CAMACHO PÉREZ ,  Brenda Karen AGUILLÓN ESTRADA ,  Grecia Andrea CARDOSO HERNÁNDEZ ,  Octavio Israel ROJAS GARCÍA ,  Eduardo MIRAMONTES BEAS

IPC分类号： C07K14/78 ,  C07K1/36 ,  B01D61/24

CPC分类号： C07K14/78 ,  C07K1/36 ,  B01D61/243 ,  B01D2325/0283 ,  B01D2311/12

摘要： A method for producing human collagen structures with controlled characteristics, having the following stages: a) Tissue conditioning b) Pre-treatment; b1) final washings with distilled water; c) extraction by enzymatic hydrolysis by subjecting the tissue to a solution of acetic acid with pepsin; d) precipitation, where the resulting collagen solution is brought to a high concentration by adding sodium chloride and where the fibers recovered from the sieve are solubilized again in an acetic acid solution; e) dynamic dialysis to purify the solution from the excess of salt present in the collagen solution; f) lyophilization at −40° C. and a vacuum pressure of 0.04 mbar (4 Pa); g) molding or second lyophilization, where a concentration is chosen and collagen is solubilized in an acetic acid solution; once solubilized, collagen is placed in molds to generate the desired structure and once again, the solution is lyophilized; h) crosslinking, where the collagen pieces are subjected to a formaldehyde vapor atmosphere in a crosslinking apparatus and i) pressing, where the collagen structure is subjected to a mechanical force of 400-5000 N compacting to a value of 0.01-10 mm and increasing its fibrillar density.