公开(公告)号：US20170073758A1

公开(公告)日：2017-03-16

申请号：US14855719

申请日：2015-09-16

申请人： Janssen Diagnostics LLC

发明人： Yixin Wang ,  Abhijt Mazumder ,  Dmitri Talantov ,  Timothy Jatkoe ,  Jonathan Baden

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16

摘要： The present invention provides a method of identifying origin of a metastasis of unknown origin by obtaining a sample containing metastatic cells; measuring Biomarkers associated with at least two different carcinomas; combining the data from the Biomarkers into a linear discrimination analysis where the linear discrimination analysis normalizes the Biomarkers against a reference; and imposes a cut-off which optimizes sensitivity and specificity of each Biomarker, weights the prevalence of the carcinomas and selects a tissue of origin determining origin based on highest probability determined by the linear discrimination analysis or determining that the carcinoma is not derived from a particular set of carcinomas; and optionally measuring Biomarkers specific for one or more additional different carcinoma, and repeating the steps for additional Biomarkers.

摘要翻译： 本发明提供了通过获得含有转移细胞的样品来鉴定未知来源的转移起点的方法; 测量与至少两种不同癌相关的生物标志物; 将来自生物标志物的数据组合成线性鉴别分析，其中线性鉴别分析使生物标志物反对参考标准化; 并强加了一个优化每个生物标志物的灵敏度和特异性的切断点，重视癌症的流行，并且基于通过线性鉴别分析确定的最高概率来选择确定起源的组织，或者确定癌症不是从特定的 一套癌; 并且任选地测量对一种或多种另外的不同癌特异性的生物标志物，并重复其它生物标志物的步骤。