公开(公告)号：US20090258367A1

公开(公告)日：2009-10-15

申请号：US12416033

申请日：2009-03-31

Applicant: JOON WON PARK ,  YU JIN JUNG ,  YU SHIN PARK ,  HONG GIL NAM

Inventor： JOON WON PARK ,  YU JIN JUNG ,  YU SHIN PARK ,  HONG GIL NAM

IPC: C12Q1/68

CPC classification number: G01Q60/42 ,  C12Q1/6841 ,  C12Q2565/601 ,  C12Q2565/507

Abstract: The present invention relates to a method of mapping of mRNA distribution, comprising the steps of a preparing a probe DNA attached to a apical liner region of the dendron on AFM cantilever where the probe DNA can specifically hybridize a target mRNA and measuring specific adhesive force between the probe DNA and the target mRNA on sectioned tissue at nanometer resolution.

Abstract translation: 本发明涉及一种映射mRNA分布的方法，包括以下步骤：制备连接到AFM悬臂上的树突的顶端衬里区域上的探针DNA，其中探针DNA可以特异性地杂交目标mRNA并测量其间的特异性粘附力 探针DNA和靶mRNA在纳米分辨率的切片组织上。