公开(公告)号：US20230276832A1

公开(公告)日：2023-09-07

申请号：US18008191

申请日：2022-01-11

Applicant: JIANGSU UNIVERSITY

Inventor： Haile MA ,  Xiaofeng REN ,  Qiufang LIANG ,  Xinru SUN ,  Xueqiong LIU ,  Jialin TANG ,  Chengwei ZHOU

IPC: A23L5/30 ,  A23L33/18 ,  A23L33/105 ,  A23L29/275

CPC classification number: A23L5/32 ,  A23L33/18 ,  A23L33/105 ,  A23L29/275 ,  A23V2002/00

Abstract: An ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components is conducted as follows: Chitosan solution containing 1% glacial acetic acid is added gently to the casein phosphopeptide solution containing quercetin stock solution in an equal volume under constant magnetic stirring; pH is adjusted accordingly before subjecting the mixture solution to ultrasonic treatment; after ultrasonication, the quercetin-loaded casein phosphopeptide-chitosan nanoparticles are obtained by freeze-drying. In the process of using the electrostatic interaction between the casein phosphopeptide and the chitosan to embed the quercetin, the present disclosure employed the multi-mode ultrasonic processing technology to facilitate the cross-linking of the polypeptide and the polysaccharide through the physical force of ultrasound. This enabled the complex encapsulation to incorporate additional bioactive components. The quercetin product exhibited excellent encapsulation efficiency, good water solubility, good light and thermal stability, and strong antioxidant properties, which significantly expanded the bioavailability of quercetin in the gastrointestinal tract.