公开(公告)号：US20030180763A1

公开(公告)日：2003-09-25

申请号：US10334488

申请日：2002-12-30

申请人： Innogenetics N.V.

发明人： Ilse De Canck ,  Guy Mersch ,  Rudi Rossau

IPC分类号： C12Q001/68 ,  C12P019/34

CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The present invention relates to the typing of HLA alleles. The sequence of exon 2 and exon 3 of the alleles HLA-B*3913, HLA-B*1406, and HLA-B*51new and of exon 2 of the alleles HLA-DRB1*0820, HLA-DRB1*04new and HLA-DRB4*01new are disclosed. The present invention relates to methods for typing of said alleles. According to a preferred embodiment, said typing comprises the following steps: i) amplifying a relevant fragment of said alleles using at least one suitable pair of primers; ii) hybridizing the amplification product of step i) to at least one probe that specifically hybridizes to a target region comprising one or more polymorphic nucleotides in said relevant fragment; iii) determining from the result of step ii) the absence or presence of said alleles in the sample. The present invention further provides primers and probes to be used in said methods for typing. A diagnostic kit comprising said primers and probes is also part of the present invention.

摘要翻译： 本发明涉及HLA等位基因的分型。 等位基因HLA-B * 3913，HLA-B * 1406和HLA-B * 51新的外显子2和外显子3的序列和等位基因HLA-DRB1 * 0820，HLA-DRB1 * 04新和HLA- DRB4 * 01新公开。 本发明涉及所述等位基因的分型方法。 根据优选实施方案，所述分型包括以下步骤：i）使用至少一对合适的引物扩增所述等位基因的相关片段; ii）将步骤i）的扩增产物杂交至至少一种与所述相关片段中包含一个或多个多核苷酸的靶区域特异性杂交的探针; iii）从步骤ii）的结果确定样品中不存在或存在所述等位基因。 本发明还提供了用于所述打字方法的引物和探针。 包含所述引物和探针的诊断试剂盒也是本发明的一部分。