公开(公告)号：US12215350B2

公开(公告)日：2025-02-04

申请号：US16796226

申请日：2020-02-20

Applicant: ImmunityBio, Inc.

Inventor： Rohit Duggal ,  Ranjeet Sinha ,  Wenzhao Li ,  Jason Isaacson ,  Karl Marquez ,  Patrick Soon-Shiong

IPC: C12N5/0783 ,  C07K14/54 ,  C07K14/735

Abstract: Cord blood or peripheral blood NK cells are prepared from whole blood mononuclear cells without the need to isolate CD34+ hematopoietic stem cells or NK cells, and without the need for a feeder layer. Advantageously, the methods presented herein use an enrichment process that uses antiCD16 agonist antibodies, antiCD3 antibodies, and N-803. Moreover, contemplated processes are suitable for adaptation into a fully automated production process (GMP in a box).

公开(公告)号：US11453862B2

公开(公告)日：2022-09-27

申请号：US16505528

申请日：2019-07-08

Applicant: ImmunityBio, Inc.

Inventor： Rohit Duggal ,  Ranjeet Sinha ,  Wenzhao Li ,  Jason Isaacson ,  Karl Marquez ,  Patrick Soon-Shiong

IPC: C12N5/0783 ,  A61K39/00

Abstract: Cord blood or peripheral blood NK cells are prepared from whole blood mononuclear cells without the need to isolate CD34+ hematopoietic stem cells or NK cells, and without the need for a feeder layer. Advantageously, the methods presented herein use an enrichment process that uses antiCD16 agonist antibodies, antiCD3 antibodies, and N-803. Moreover, contemplated processes are suitable for adaptation into a fully automated production process (GMP in a box).

公开(公告)号：US20210361711A1

公开(公告)日：2021-11-25

申请号：US17375985

申请日：2021-07-14

Applicant: ImmunityBio, Inc.

Inventor： Rohit Duggal ,  Ranjeet Sinha ,  Wenzhao Li ,  Jason Isaacson ,  Patrick Soon-Shiong

IPC: A61K35/17 ,  C12N5/0783 ,  C07K14/54 ,  C07K14/735

Abstract: Cytokine induced memory like (CIML) NK cells with enhanced cytotoxicity are presented. Most typically, the CIML NK cells are derived from a mononuclear cell fraction of peripheral blood or cord blood. In further contemplated aspects, the CIML NK cells are expanded and induced in a contained and automated production environment that substantially reduces operational complexity and production cost.