公开(公告)号：US20160215270A1

公开(公告)日：2016-07-28

申请号：US15023484

申请日：2014-09-24

Applicant: ID PHARMA CO., LTD.

Inventor： Hiroshi BAN ,  Akihiro IIDA ,  Mamoru HASEGAWA

IPC: C12N5/074 ,  A61K35/545 ,  C12N7/00

CPC classification number: C12N5/0696 ,  A61K35/545 ,  C12N7/00 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/09 ,  C12N2510/00 ,  C12N2523/00 ,  C12N2760/18843 ,  C12N2830/00 ,  C12N2840/00

Abstract: The present invention provides methods for improving the efficiency of inducing pluripotent stem cells, as well as vectors and compositions for use therein. In the induction of pluripotent stem cells which contains the step of introducing a vector that contains the KLF gene, OCT gene, and SOX gene in this order, the efficiency of pluripotent stem cell induction was successfully increased significantly by further introducing a vector that contains the KLF gene but not the OCT gene and the SOX gene. The methods of the present invention have an excellent feature in that they allow efficient induction of pluripotent stem cells under a temperature condition closer to the physiological environment, and prompt vector removal after the pluripotent stem cell induction. The present invention enables more efficient induction of pluripotent stem cells.

Abstract translation: 本发明提供了提高诱导多能干细胞的效率的方法，以及用于其中的载体和组合物。 在诱导含有KLF基因，OCT基因和SOX基因的载体的多能干细胞的诱导中，多能干细胞诱导的效率通过进一步引入含有 KLF基因，但不是OCT基因和SOX基因。 本发明的方法具有优异的特征，它们允许在更接近生理环境的温度条件下有效诱导多能干细胞，并在多能干细胞诱导后迅速进行载体移除。 本发明能够更有效地诱导多能干细胞。

公开(公告)号：US20160177337A1

公开(公告)日：2016-06-23

申请号：US15063929

申请日：2016-03-08

Applicant: ID Pharma Co., Ltd.

Inventor： Noemi FUSAKI ,  Hiroshi BAN ,  Mamoru HASEGAWA ,  Yoshikazu YONEMITSU

IPC: C12N15/86 ,  C12N7/00 ,  C12N5/074

CPC classification number: C12N15/86 ,  C07K14/4702 ,  C12N5/0696 ,  C12N7/00 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2760/18622 ,  C12N2760/18643 ,  C12N2760/18645 ,  C12N2760/18662 ,  C12N2760/18822 ,  C12N2760/18843

Abstract: An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.