公开(公告)号：US06197545B1

公开(公告)日：2001-03-06

申请号：US09269345

申请日：1999-03-25

申请人： Hong Fang ,  Neil Green ,  Luc Van Kaer

发明人： Hong Fang ,  Neil Green ,  Luc Van Kaer

IPC分类号： C12N1581

CPC分类号： C12N9/52 ,  C12N15/81

摘要： The present invention provides a yeast cell comprising a nucleic acid functionally encoding a eukaryotic (e.g., mammalian) protein homologue of a subunit (Sec11p, Spc1p, Spc2p and/or Spc3p) of the yeast signal peptidase complex. The yeast cell of this invention can be, for example, of the genus Saccharomyces, Schizosaccharomyces, Pichia, Hansenula, Kluyveromyces and/or Yarrowia. Furthermore, the yeast cell can lack one or more functional subunits (Sec11p, Spc1p, Spc2p and/or Spc3p) of the yeast signal peptidase complex. The present invention further provides a method for producing a protein heterologous to a yeast cell comprising expressing, in the yeast cell of this invention, a nucleic acid functionally encoding the heterologous protein under conditions which permit the expression of the nucleic acid as a precursor protein having a signal peptide and processing of the precursor protein to a signal peptide-cleaved form of the protein.

摘要翻译： 本发明提供了酵母细胞，其包含功能性编码酵母信号肽酶复合物的亚基（Sec11p，Spc1p，Spc2p和/或Spc3p）的真核（例如哺乳动物）蛋白质同源物的核酸。 本发明的酵母细胞可以是例如酵母属，裂殖酵母属，毕赤酵母，汉逊酵母，克鲁维酵母和/或耶氏酵母属。 此外，酵母细胞可以缺乏酵母信号肽酶复合物的一个或多个功能性亚基（Sec11p，Spc1p，Spc2p和/或Spc3p）。 本发明还提供了用于产生与酵母细胞异源的蛋白质的方法，包括在本发明的酵母细胞中表达在允许以表达核酸作为前体蛋白的条件下功能性编码异源蛋白质的核酸， 信号肽和前体蛋白加工成信号肽切割形式的蛋白质。