公开(公告)号：US20170296649A1

公开(公告)日：2017-10-19

申请号：US15326940

申请日：2015-07-17

申请人： Hiroshi KAWAMOTO

发明人： Hiroshi Kawamoto ,  Kyoko Masuda ,  Takuya Maeda ,  Seiji Nagano ,  Yoshimoto Katsura

IPC分类号： A61K39/245 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/245 ,  A61K35/17 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/5158 ,  A61K2039/585 ,  C12N7/00 ,  C12N2710/16234

摘要： Provided is a method for inducing T cells for a cell-based immunotherapy, which comprises the steps of: (1) providing human pluripotent stem cells bearing a T cell receptor specific for a desired antigen, and (2) inducing T cell progenitors or mature T cells from the pluripotent stem cells of step (1). Especially, a method for inducing T cells for a cell-based immunotherapy from cells of a person who is not the subject to be treated by the cell-based immunotherapy. The method provided herein may further comprise a step of co-culturing the T cell progenitors or mature T cells induced from the pluripotent stem cells with the lymphocytes of the subject to be treated by the cell based immunotherapy to verify that the T cells are not allogenicaly reactive against the subject.

公开(公告)号：US20170267972A1

公开(公告)日：2017-09-21

申请号：US15326968

申请日：2015-07-17

申请人： Hiroshi Kawamoto

发明人： Hiroshi Kawamoto ,  Shin Kaneko ,  Kyoko Masuda ,  Takuya Maeda ,  Seiji Nagano ,  Yoshimoto Katsura

IPC分类号： C12N5/0783 ,  A61K39/00

CPC分类号： C12N5/0638 ,  A61K35/17 ,  A61K39/0011 ,  A61K2039/5158 ,  A61K2039/585 ,  A61P31/12 ,  A61P35/00 ,  A61P37/08 ,  C12N15/09 ,  C12N2502/1121 ,  C12N2506/45

摘要： Provided is a method for inducing T cells for use in a cell-based immunotherapy, comprising the steps of:(1) providing human pluripotent stem cells bearing a T cell receptor specific for a WT1 antigen or an Epstein-Barr virus associated antigen, and (2) inducing T cell progenitors or mature T cells from the pluripotent stem cells provided in step (1). Pluripotent stem cells may preferably be iPS cells. The human pluripotent stem cells bearing a T cell receptor specific for an antigen may be prepared by inducing iPS cells from a T cell having the desired antigen specificity or by introducing genes encoding the T cell receptor specific for the desired antigen into iPS cells. The T cells obtained by this method can be used for the treatment of various immune-related diseases such as cancers and infectious diseases.