公开(公告)号：US09765379B2

公开(公告)日：2017-09-19

申请号：US14497964

申请日：2014-09-26

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Richard St. John ,  Jane V. Gunson ,  Kimberly Kaleas ,  Deepa Nadarajah ,  Rachel L E Adams ,  Bradley R. Snedecor

IPC: C12P21/00 ,  C07K16/00

CPC classification number: C12P21/00 ,  C07K16/00

Abstract: The present invention contemplates methods of producing a recombinant protein comprising fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. Furthermore, method of producing a recombinant protein comprising fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, wherein the recombinant protein yield is increased by about 20% or greater is contemplated.