公开(公告)号：US20240084375A1

公开(公告)日：2024-03-14

申请号：US18495615

申请日：2023-10-26

Applicant: Genentech, Inc.

Inventor： Christian N. Cunningham ,  Kenneth Karl Hallenbeck

IPC: C12Q1/686 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/1805

Abstract: In one embodiment, a method and system normalize a polymerase chain reaction (PCR) amplification process for a plurality of samples containing DNA. Cycle data for performing the PCR amplification process is identified for the plurality of samples. The cycle data includes a corresponding cycle count for each sample of the plurality of samples. The plurality of samples is sorted into a plurality of bins based on the cycle data such that cycle count variation between bins of the plurality of bins is reduced. A bin cycle count is assigned to each bin of the plurality of bins. The bin cycle count is unique to each bin of the plurality of bins. Identification information is generated for the bins. An output for performing a PCR amplification of the plurality of samples is generated using the bins and the bin cycle count for each bin of the plurality of bins.

公开(公告)号：US11834709B2

公开(公告)日：2023-12-05

申请号：US17502022

申请日：2021-10-14

Applicant: Genentech, Inc.

Inventor： Christian N. Cunningham ,  Kenneth Karl Hallenbeck

IPC: C12Q1/68 ,  C12Q1/686 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/1805

Abstract: A method and system for normalizing a polymerase chain reaction (PCR) amplification process for a plurality of samples containing DNA. Cycle data for performing the PCR amplification process is identified for the plurality of samples. The cycle data includes a corresponding cycle count for each sample of the plurality of samples. The plurality of samples is sorted into a plurality of bins based on the cycle data such that cycle count variation between bins of the plurality of bins is reduced. A bin cycle count is assigned to each bin of the plurality of bins. The bin cycle count is unique to each bin of the plurality of bins. Identification information is generated for the bins. An output for performing a PCR amplification of the plurality of samples is generated using the bins and the bin cycle count for each bin of the plurality of bins.