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公开(公告)号：US20020142467A1
公开(公告)日：2002-10-03
申请号：US10131053
申请日：2002-04-22
Applicant: Genentech, Inc.
Inventor： Jennie P. Mather , Axel Ullrich
IPC: C12P021/02 , C12N005/06 , C12N015/87
Abstract: A method for culturing a recombinant host cell comprising: determining a polypeptide factor for a polypeptide factor-dependent host cell; transforming said host cell with nucleic acid encoding said polypeptide factor; transforming the host cell with nucleic acid encoding a desired protein; and, culturing the transformed host cells in a medium lacking the polypeptide factor.
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