公开(公告)号：US20240240175A1

公开(公告)日：2024-07-18

申请号：US18411697

申请日：2024-01-12

Applicant: GENENTECH, INC.

Inventor： Shahram Misaghi ,  Danming Tang ,  Brad Snedecor

IPC: C12N15/10

CPC classification number: C12N15/1082 ,  C12N15/1055 ,  C12N15/1072

Abstract: The presently disclosed subject matter relates to combinatorial vector cloning and transfection strategies for the generation of targeted integration host cells suitable for the expression of recombinant proteins, as well as targeted integration host cells generated by said strategies and compositions comprising said targeted integration host cells.

公开(公告)号：US20240190944A1

公开(公告)日：2024-06-13

申请号：US18514280

申请日：2023-11-20

Applicant: Genentech, Inc.

Inventor： Shahram MISAGHI ,  Brian Megia Castellano ,  Danming Tang ,  Avi J. Ashkenazi ,  Bradley R. Snedecor

IPC: C07K16/00 ,  C07K14/47 ,  C12N9/12 ,  C12N15/90

CPC classification number: C07K16/00 ,  C07K14/4747 ,  C12N9/12 ,  C12N15/907 ,  C12Y207/11001 ,  C07K2317/14 ,  C12N2310/20

Abstract: The present disclosure relates to cells (e.g., Chinese Hamster Ovary (CHO) cells) that are modified to reduce or eliminate the expression of certain endogenous cellular proteins, and methods of using such cells in the production of a recombinant product of interest, e.g., a recombinant protein, a recombinant viral particle, or a recombinant viral vector. These modifications were specifically chosen to generate engineered cells with desired traits in several key areas, including improved cell culture performance (e.g., higher viability and product titers).