公开(公告)号：US11781185B2

公开(公告)日：2023-10-10

申请号：US17947984

申请日：2022-09-19

申请人： Element Biosciences, Inc.

发明人： Sinan Arslan ,  Molly He ,  Michael Previte ,  Ramreddy Tippana ,  Hua Yu ,  William Light ,  Junhua Zhao

IPC分类号： C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806

CPC分类号： C12Q1/6874 ,  C12Q1/6806

摘要： Provided herein are fluorescently-labeled nucleotide conjugates for nucleic acid analysis. Also provided are reagents used for forming binding complexes between a fluorescently-labeled nucleotide conjugate and a target nucleic acid sequence in the presence of one or more reagents disclosed herein. Binding complexes can be detected in the presence of the one or more reagents. For example, the one or more reagents may contain a photobleaching reducing agent configured to reduce photobleaching resulting from use of the fluorescently-labeled nucleotide conjugate to form the binding complex in a nucleic acid analysis. Such nucleic acid analysis may be used to identify sites of nucleobase binding or incorporation between the target nucleic acid sequence and one or more nucleotide moieties of the fluorescently-labeled nucleotide conjugate in a nucleic acid sequence reaction.