公开(公告)号：US20170037417A9

公开(公告)日：2017-02-09

申请号：US14167292

申请日：2014-01-29

Applicant: Dyadic International, Inc.

Inventor： Mark A. EMALFARB ,  Peter J. PUNT ,  Cornelia VAN ZEIJL ,  Cornelius VAN DEN HONDEL ,  Jan Cornelis VERDOES ,  Richard P. BURLINGAME

IPC: C12N15/80 ,  G01N33/561 ,  C12P21/00

CPC classification number: C12N15/80 ,  C12P21/00 ,  C40B40/06 ,  G01N33/561

Abstract: The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

Abstract translation: 本发明通常涉及修饰的丝状真菌宿主细胞，其包含编码一种或多种在一种或多种在所述细胞中起作用的启动子控制的多肽的核酸。 还公开了使用修饰细胞表达一种或多种多肽的方法，包括筛选用一种或多种包含衍生自包括cDNA的合成或基因组核酸的核酸的表达载体转化的细胞的方法。 还公开了纯化一种或多种包含在修饰细胞中表达的多肽或复合物的多肽或复合物的方法，其用作结构/功能研究中的底物，作为治疗剂，作为诊断试剂，或作为人或动物疫苗。

公开(公告)号：US20150211013A1

公开(公告)日：2015-07-30

申请号：US14167292

申请日：2014-01-29

Applicant: Dyadic International, Inc.

Inventor： Mark A. EMALFARB ,  Peter J. PUNT ,  Cornelia VAN ZEIJL ,  Cornelius VAN DEN HONDEL ,  Jan Cornelis VERDOES ,  Richard P. BURLINGAME

IPC: C12N15/80 ,  G01N33/561 ,  C12P21/00

CPC classification number: C12N15/80 ,  C12P21/00 ,  C40B40/06 ,  G01N33/561

Abstract: The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

Abstract translation: 本发明通常涉及修饰的丝状真菌宿主细胞，其包含编码一种或多种在一种或多种在所述细胞中起作用的启动子控制的多肽的核酸。 还公开了使用修饰细胞表达一种或多种多肽的方法，包括筛选用一种或多种包含衍生自包括cDNA的合成或基因组核酸的核酸的表达载体转化的细胞的方法。 还公开了纯化一种或多种包含在修饰细胞中表达的多肽或复合物的多肽或复合物的方法，其用作结构/功能研究中的底物，作为治疗剂，作为诊断试剂，或作为人或动物疫苗。