公开(公告)号：US08871493B2

公开(公告)日：2014-10-28

申请号：US13621507

申请日：2012-09-17

Applicant: Dyadic International, Inc.

Inventor： Mark Aaron Emalfarb ,  Richard Paul Burlingame ,  Philip Terry Olson ,  Arkady Panteleimonovich Sinitsyn ,  Martine Parriche ,  Jean Christophe Bousson ,  Christine Marie Pynnonen ,  Peter Jan Punt ,  Cornelia Marie Johanna Van Zeijl

IPC: C12N1/15 ,  C12N15/80 ,  C12N9/36 ,  C12N9/58 ,  C12N9/42 ,  C12N9/24

CPC classification number: C12N15/80 ,  C12N9/2437 ,  C12N9/2462 ,  C12N9/2482 ,  C12N9/58 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01091

Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Abstract translation: 描述了用于表达和分泌异源蛋白质或多肽的丝状真菌宿主领域的新型转化系统。 本发明还涵盖以经济方式生产大量多肽或蛋白质的方法。 该系统包括转化或转染的Chrysosporium属的真菌菌株，更特别是Chrysosporium lucknowense及其突变体或衍生物。 它还包括含有Chrysosporium编码序列的转化体，以及Chrysosporium基因的表达调节序列。 还提供了新颖的真菌酶及其编码序列和表达调节序列。