公开(公告)号：US09382523B2

公开(公告)日：2016-07-05

申请号：US14536016

申请日：2014-11-07

Applicant: Dupont Nutrition Biosciences APS

Inventor： Susan Madrid ,  Cherry Lin ,  Masoud Rajabi Zargahi ,  Rikke Høegh Lorentsen ,  Mai Faurschou Isaksen ,  Michael Ward

IPC: C12N15/80 ,  C12N9/20 ,  A23K1/165 ,  A23L1/03 ,  A23L1/30

CPC classification number: C12N9/20 ,  A23K20/189 ,  A23L29/06 ,  A23L33/14 ,  A23V2002/00 ,  C12N15/80 ,  C12Y301/01003

Abstract: The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.

Abstract translation: 本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中 核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码脂肪分解酶的异源核苷酸序列的条件下培养细胞。