公开(公告)号：US11972620B2

公开(公告)日：2024-04-30

申请号：US17076008

申请日：2020-10-21

Applicant: Cytek Biosciences, Inc.

Inventor： Alan Li ,  Shobana Vaidyanathan

IPC: G06V20/69 ,  G01N15/14 ,  G06T7/00 ,  G06T7/11

CPC classification number: G06V20/698 ,  G01N15/1475 ,  G06T7/0012 ,  G06T7/11 ,  G06V20/693 ,  G06V20/695 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06V2201/03

Abstract: A classifier engine provides cell morphology identification and cell classification in computer-automated systems, methods and diagnostic tools. The classifier engine performs multispectral segmentation of thousands of cellular images acquired by a multispectral imaging flow cytometer. As a function of imaging mode, different ones of the images provide different segmentation masks for cells and subcellular parts. Using the segmentation masks, the classifier engine iteratively optimizes model fitting of different cellular parts. The resulting improved image data has increased accuracy of location of cell parts in an image and enables detection of complex cell morphologies in the image. The classifier engine provides automated ranking and selection of most discriminative shape based features for classifying cell types.